CD133 Antibody (2F8C5) - BSA Free

Novus Biologicals | Catalog # NBP2-52473

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F8C5

Format

BSA Free
View all CD133 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD133 (AA: 20-108) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD133 Antibody (2F8C5) - BSA Free

Western Blot: CD133 Antibody (2F8C5)BSA Free [NBP2-52473]

Western Blot: CD133 Antibody (2F8C5)BSA Free [NBP2-52473]

Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against HEK293 (1) and PROM1-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD133 Antibody (2F8C5) - BSA Free [NBP2-52473]

Flow Cytometry: CD133 Antibody (2F8C5) - BSA Free [NBP2-52473]

Flow Cytometry: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis of Hela cells using PROM1 mouse mAb (green) and negative control (red).
Western Blot: CD133 Antibody (2F8C5)BSA Free [NBP2-52473]

Western Blot: CD133 Antibody (2F8C5)BSA Free [NBP2-52473]

Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against human PROM1 (AA: 20-108) recombinant protein. (Expected MW is 36.1 kDa)
ELISA: CD133 Antibody (2F8C5) - BSA Free [NBP2-52473]

ELISA: CD133 Antibody (2F8C5) - BSA Free [NBP2-52473]

ELISA: CD133 Antibody (2F8C5) [NBP2-52473] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for CD133 Antibody (2F8C5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (2F8C5) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD133 Antibody (2F8C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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