CD133-B19, 5'-DY647

Catalog # Availability Size / Price Qty
CD133-B19, 5'-DY647 | Fluorescent Cell Surface Markers
1 Image
Description: High affinity fluorescently tagged aptamer for CD133 (extracellular domain epitope)
Product Details

Biological Activity

High affinity fluorescently tagged aptamer for CD133 (extracellular domaine epitope) (Kd values are 52.3 and 145.1 nM in Hep3B and HT29 cells, respectively). Efficiently internalized upon binding to CD133. Exhibits superior penetration of tumorspheres compared with an antibody to the same target.

For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.

Technical Data

Aptamer type (DNA/RNA)
Number of Bases
Sequence Modifications
2-fluoropyrimidines, 3'-inverted deoxythymidine cap, 5'-fluorescent DY647 tag
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the patent holder, Deakin University.

Background References

  1. RNA aptamers targeting cancer stem cell marker CD133.
    Shigdar et al.
    Cancer Lett., 2013;330:84

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).


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