CD14 Antibody (503) - Azide and BSA Free

Name: CD14 Antibody (503) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-90362
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-90362

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 503 expressed in HEK293

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 (Accession#: NP_033971.1; Met1-Pro345).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD14 Antibody (503) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (503) [NBP2-90362]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (503) [NBP2-90362] - Staining of Mouse CD14 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD14 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.

Flow Cytometry: CD14 Antibody (503) [NBP2-90362]

Flow Cytometry: CD14 Antibody (503) [NBP2-90362] - Analysis of Mouse CD14 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse CD14, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for CD14 Antibody (503) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:25-1:100

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (503) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for CD14 Antibody (503) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.