CD155/PVR Antibody (D171)

Novus Biologicals | Catalog # NB600-1241

Clone D171 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Mouse, Primate

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # D171
View all CD155/PVR Primary Antibodies »

Product Specifications

Immunogen

HeLa cells

Epitope

Amino acids 35 - 50.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cell membrane.

Specificity

Polio Virus Receptor (D171)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD155/PVR Antibody (D171)

Western Blot: CD155/PVR Antibody (D171) [NB600-1241]

Western Blot: CD155/PVR Antibody (D171) [NB600-1241]

Western Blot: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of 25 ug of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241]

Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241]

Immunocytochemistry/Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of CD155 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241]

Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241]

Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of CD155 in Hela cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241]

Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241]

Immunocytochemistry/Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of CD155 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241]

Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241]

Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of U937 cells compared to an isotype control (blue).

Applications for CD155/PVR Antibody (D171)

Application
Recommended Usage

Flow Cytometry

2 ug

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Western Blot

1:10 - 1:100
Application Notes
WB: not suitable. This antibody protects HeLa cells against thecytopathic effect of all three poliovirus serotypes. Intact Ig or Fab fragment of this antibody prevents binding of 35S-labeled poliovirus to HeLa cells.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4) and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD155/PVR

Polio Virus Receptor (PVR) is a member of Ig-superfamily with 3 Ig-domains in arrangement V-C-C. Four mRNAs are produced from single gene for PVR, (mapped to human chromosome band 19q13.1-13.2). Two of the mRNAs (called H20A and H20B or alpha and delta) encode cell surface molecules of about 43kDa and 45kDa polypeptide backbone, respectively. Two other splice variants, beta and gamma encode secreted molecules of about 44kDa. The cytoplasmic tails are short (aa 35-50) and are rich in serine phosphorylated residues.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat)

Gene Symbol

PVR

Additional CD155/PVR Products

Product Documents for CD155/PVR Antibody (D171)

Certificate of Analysis

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Product Specific Notices for CD155/PVR Antibody (D171)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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