CD1a Antibody (O10+C1A/711) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34698

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # O10+C1A/711

Format

Azide and BSA Free
View all CD1a Primary Antibodies »

Product Specifications

Immunogen

Human thymus cells (O10); Recombinant human CD1a protein (C1A/711)

Localization

Cell surface

Marker

Mature Langerhans Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG1 Kappa

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34314).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD1a Antibody (O10+C1A/711) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698]

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698]

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10+C1A/711).
Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698]

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698]

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698] - Paraffin-embedded human tonsil epithelium. Image from verified customer review.

Applications for CD1a Antibody (O10+C1A/711) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-34698 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD1a

At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).

Long Name

T Cell Surface Glycoprotein CD1a

Alternate Names

CD1a, FCB6, HTA1, R4, T6

Gene Symbol

CD1A

Additional CD1a Products

Product Documents for CD1a Antibody (O10+C1A/711) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD1a Antibody (O10+C1A/711) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD1a Antibody (O10+C1A/711) - Azide and BSA Free (1)

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  • CD1a Antibody (O10+C1A/711)
    Name: Anonymous
    Application: Immunofluorescence - paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 09/23/2018
    CD1a human tonsil epithelium
    pH9 antigen retrieval
    CD1a Antibody (O10+C1A/711) - Azide and BSA Free NBP2-34698

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail