Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Sheep IgG
View all CD1d1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD1d1
Gln22-Gly305
Accession # NP_031665

Specificity

Detects mouse CD1d1 in direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant mouse CD1d2 is observed, and approximately 15% cross-reactivity with recombinant human CD1d1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for CD1d1 Antibody [CoraFluor™ 1]

CD1d1 Antibody [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for CD1d1 Antibody [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD1d1

CD1d1 is a 48 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. Complexes of CD1d1 with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes. CD1d1-presented glycolipids are recognized by canonical NKT cells that utilize an invariant mouse V alpha 14-J alpha 18 chain in their T cell receptor (V alpha 24-J alpha 18 in human). The interaction with glycolipid-loaded CD1d1 is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines. In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d1 in antigen presenting cells.

Alternate Names

AI747460, CD1.1, CD1A, Cd1d, Ly-38

Gene Symbol

Cd1d1

Additional CD1d1 Products

Product Documents for CD1d1 Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD1d1 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail