CD24 Antibody (DMC213) - Chimeric

Novus Biologicals | Catalog # NBP3-48290

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC213
View all CD24 Primary Antibodies »

Product Specifications

Immunogen

CD24 recombinant protein expressed in mammalian cells (Accession# P25063). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for CD24 Antibody (DMC213) - Chimeric

CD24 Antibody (DMC213) - Chimeric

Flow Cytometry: CD24 Antibody (DMC213) - Chimeric [NBP3-48290]

Flow cytometry analysis with Anti-CD24 (DMC213) on HEK293 cells transfected with human CD24 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
CD24 Antibody (DMC213) - Chimeric

Immunohistochemistry: CD24 Antibody (DMC213) - Chimeric [NBP3-48290]

A. NBP3-48290 at 10ug/ml staining CD24 in K562-CD24 cells by IHC (Catalog Number# NBP3-48290);B. NBP3-48290 at 10ug/ml staining CD24 in human tonsil tissue by IHC (Catalog Number# NBP3-48290)
CD24 Antibody (DMC213) - Chimeric

Flow Cytometry: CD24 Antibody (DMC213) - Chimeric [NBP3-48290]

Flow cytometry analysis of antigen binding of anti-human CD24 mAb(NBP3-48290). (A) NBP3-48290 does not bind to Jurkat cells that do not express CD24. (B) A clear peak shift of NBP3-48290 was seen compared to the control when incubated with CD24-expressing MCF-7 cells, indicating strong binding of NBP3-48290 to CD24. Antibodies were incubated at 10 ug/mL.
CD24 Antibody (DMC213) - Chimeric

Western Blot: CD24 Antibody (DMC213) - Chimeric [NBP3-48290]

Anti-CD24 antibody (Catalog Number# NBP3-48290) at 1/1000 dilutionLane : Huh7, whole cell lysateSecondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilutionPredicted band size: 61 kDaObserved band size: 25, 135 kDa

Applications for CD24 Antibody (DMC213) - Chimeric

Application
Recommended Usage

ELISA

1:5000-10000

Flow Cytometry

1:100

Immunohistochemistry

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD24

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Alternate Names

CD24, CD24A

Gene Symbol

CD24

Additional CD24 Products

Product Documents for CD24 Antibody (DMC213) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD24 Antibody (DMC213) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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