![Western Blot: CD2AP Antibody [NBP2-53098]](https://resources.rndsystems.com/images/products/CD2AP-Antibody-Western-Blot-NBP2-53098-img0008.jpg "Western Blot: CD2AP Antibody [NBP2-53098]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (90%), Porcine (92%), Rhesus Macaque (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton, Cell projection, ruffle
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit CD2AP Antibody (NBP2-53098) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD2AP Antibody
Western Blot: CD2AP Antibody [NBP2-53098]
Western Blot: CD2AP Antibody [NBP2-53098] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD2AP antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098]](https://resources.rndsystems.com/images/products/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP2-53098-img0006.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098]")
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098]
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098] - Mouse colon. CD2AP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: CD2AP Antibody [NBP2-53098]](https://resources.rndsystems.com/images/products/CD2AP-Antibody-Western-Blot-NBP2-53098-img0002.jpg "Western Blot: CD2AP Antibody [NBP2-53098]")
Western Blot: CD2AP Antibody [NBP2-53098]
Western Blot: CD2AP Antibody [NBP2-53098] - CD2AP antibody detects CD2AP protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE CD2AP antibody dilution: 1:1000![Western Blot: CD2AP Antibody [NBP2-53098]](https://resources.rndsystems.com/images/products/CD2AP-Antibody-Western-Blot-NBP2-53098-img0004.jpg "Western Blot: CD2AP Antibody [NBP2-53098]")
Western Blot: CD2AP Antibody [NBP2-53098]
Western Blot: CD2AP Antibody [NBP2-53098] - A. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE CD2AP antibody dilution: 1:1000![Immunocytochemistry/ Immunofluorescence: CD2AP Antibody [NBP2-53098]](https://resources.rndsystems.com/images/products/CD2AP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53098-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD2AP Antibody [NBP2-53098]")
Immunocytochemistry/ Immunofluorescence: CD2AP Antibody [NBP2-53098]
Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP2-53098] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098]](https://resources.rndsystems.com/images/products/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP2-53098-img0005.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098]")
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098]
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098] - FaDu xenograft, using CD2-associated protein antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: CD2AP Antibody [NBP2-53098] -
CD2AP antibody detects CD2AP protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD2AP stained by CD2AP antibody (NBP2-53098) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: CD2AP Antibody [NBP2-53098] -
Sample (30 ug of whole cell lysate)A: A431
7.5% SDS PAGE
NBP2-53098 diluted at 1:1000
Applications for CD2AP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry-Paraffin
1:100 - 1:1000
Western Blot
1:500 - 1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD2AP
Long Name
CD2-associated protein
Alternate Names
CMS
Gene Symbol
CD2AP
Additional CD2AP Products
Product Documents for CD2AP Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD2AP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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