CD3 epsilon Antibody (APA1/1) - BSA Free

Novus Biologicals | Catalog # NBP1-30426

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # APA1/1

Format

BSA Free
View all CD3 epsilon Primary Antibodies »

Product Specifications

Immunogen

Purified human CD3 proteins isolated from thymus

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD3 epsilon Antibody (APA1/1) - BSA Free

Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]

Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]

Flow Cytometry: CD3 epsilon Antibody (APA1/1) [NBP1-30426] - Intracellular staining of JURKAT cells with anti-CD3 epsilon activation epitope (APA1/1) PE.
Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]

Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]

Flow Cytometry: CD3 epsilon Antibody (APA1/1) [NBP1-30426] - Intracellular staining of human peripheral blood with anti-CD3 epsilon activation epitope (APA1/1) purified, GAM-FITC.
CD3 epsilon Antibody (APA1/1) - BSA Free Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]

Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]

Separation of Jurkat cells stained anti-human CD3 activation epitope (APA1/1) purified antibody (concentration in sample 5 ug/ml, GAM FITC, red-filled) from Jurkat cells unstained by primary antibody (GAM FITC, black-dashed) in Analysis (intracellular staining).

Applications for CD3 epsilon Antibody (APA1/1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Flow Cytometry: Sample preparation - At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 ug/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. Immunocytochemistry: Fixed and permeabilized cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD3 epsilon

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (APA1/1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD3 epsilon Antibody (APA1/1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for CD3 epsilon Antibody (APA1/1) - BSA Free (NBP1-30426):

Flow Cytometry (NBP1-30426):
Positive control: Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 mg/ml)
Sample preparation:
At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS
+ 0.1% BSA for 20 min. on ice.
Wash with PBS and fix with 2% formaldehyde, 30 min on ice.
Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT.
Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice.
Incubate with fluorescence-labeled APA1/1 antibody (1-5 mg/ml) in PBS + 1% BSA + 0.03%
saponine in dark, 20 min. RT.
Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.

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FAQs

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