CD3 epsilon Antibody (BLR174J)

Novus Biologicals | Catalog # NBP3-14747

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, COMET

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR174J
View all CD3 epsilon Primary Antibodies »

Product Specifications

Immunogen

residues 139 and 189 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD3 epsilon Antibody (BLR174J)

CD3 epsilon Antibody (BLR174J)

Detection of CD3e in Mouse Spleen via seqIF™ staining on COMET™

CD3e was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Rabbit Anti-Human/Mouse CD3e, Monoclonal Antibody (Catalog # NBP3-14747) at 1:4000 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).​ Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Western Blot: CD3 epsilon Antibody (BLR174J) [NBP3-14747]

Western Blot: CD3 epsilon Antibody (BLR174J) [NBP3-14747]

Western Blot: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Rabbit anti-CD3E recombinant monoclonal antibody [BLR174J] used at 1:1000.
Immunohistochemistry: CD3 epsilon Antibody (BLR174J) [NBP3-14747]

Immunohistochemistry: CD3 epsilon Antibody (BLR174J) [NBP3-14747]

Immunohistochemistry: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Rabbit anti-CD3E recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.
Flow Cytometry: CD3 epsilon Antibody (BLR174J) [NBP3-14747]

Flow Cytometry: CD3 epsilon Antibody (BLR174J) [NBP3-14747]

Flow Cytometry: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Rabbit anti-CD3E recombinant monoclonal antibody or isotype control (unshaded).

Applications for CD3 epsilon Antibody (BLR174J)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

12 µl/1 mg lysate

Western Blot

1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (BLR174J)

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (BLR174J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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