CD30/TNFRSF8 Antibody (PSH04-10)

Western Blot: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142]

Western Blot: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32142) at 1/1,000 dilution.

Lane 1: HDLM-2 cell lysate
Lane 2: HUT 102 cell lysate
Lane 3: U-937 cell lysate (negative)
Lane 4: HL-60 cell lysate (negative)
Lane 5: Daudi cell lysate (negative)
Lane 6: HeLa cell lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 64 kDa
Observed band size: 75-120 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32142) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142]

Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32142) at 1/1,500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32142) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.