CD35 Antibody (E11) [Biotin]

Novus Biologicals | Catalog # NBP2-33080B

Clone E11 was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Species Reactivity

Human, Baboon, Cynomolgus Monkey, Monkey, Rhesus Macaque

Applications

Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # E11
View all CD35 Primary Antibodies »

Product Specifications

Immunogen

Intact human monocytes

Localization

Cell surface

Marker

Follicular Dendritic Cell Marker

Specificity

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for CD35 Antibody (E11) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in IHC-P has been reported by customer review.

Reviewed Applications

Read 1 review rated 3 using NBP2-33080B in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (E11) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD35 Antibody (E11) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation
Complement Pathway

Customer Reviews for CD35 Antibody (E11) [Biotin] (1)

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  • CD35 Antibody (E11) [Biotin]
    Name: Faye Doherty
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human kidney and liver and spleen
    Species: Human
    Verified Customer | Posted 07/01/2019
    CD35 Human Kidney Staining
    Tested in IHC application, of average quality obtained realistic results but slight background noise from the antibody.
    CD35 Antibody (E11) [Biotin] NBP2-33080B

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Protocols

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FAQs

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