CD39L4/ENTPD5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85751

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD39L4/ENTPD5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AGSTGTRIHVYTFVQKMPGQLPILEGEVFDSVKPGLSAFVDQPKQGAETVQGLLEVAKDSIPRSHWKKTPVVLKATAGLRLLPEHKAKALLFEVKEIFRKSPFLVPKGSVSIMDGSDEGILAWVTVNFLTGQLHGHRQETVGTL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (87%). Reactivity reported in scientific literature (PMID: 24435307)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD39L4/ENTPD5 Antibody - BSA Free

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Analysis in human liver and skeletal muscle tissues using NBP1-85751 antibody. Corresponding ENTPD5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751]

Immunohistochemistry-Paraffin: CD39L4/ENTPD5 Antibody [NBP1-85751] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751]

Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751]

Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751] - Simple Western lane view shows a specific band for CD39L4/ENTPD5 in 0.2 mg/ml of h. Liver lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751]

Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751]

Simple Western: CD39L4/ENTPD5 Antibody [NBP1-85751] - Electropherogram image of the corresponding Simple Western lane view. CD39L4/ENTPD5 antibody was used at 1:25 dilution on h. Liver lysate(s) respectively.
CD39L4/ENTPD5 Antibody - BSA Free Western Blot: CD39L4/ENTPD5 Antibody - BSA Free [NBP1-85751]

Western Blot: CD39L4/ENTPD5 Antibody - BSA Free [NBP1-85751]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Liver tissue

Applications for CD39L4/ENTPD5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Liver lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 57 kDa.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD39L4/ENTPD5

ENTPD5 is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 5

Alternate Names

CD39L4, ENTPD5, ER-UDPase, NTPDase 5, PCPH

Gene Symbol

ENTPD5

Additional CD39L4/ENTPD5 Products

Product Documents for CD39L4/ENTPD5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CD39L4/ENTPD5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD39L4/ENTPD5 Antibody - BSA Free

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Protocols

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