Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG1 Clone # MR-1

Format

BSA Free
View all CD40 Ligand/TNFSF5 Primary Antibodies »

Product Specifications

Immunogen

Activated mouse Th1 clone D1.6

Specificity

The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Armenian Hamster CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free (NBP1-47234) is a monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free [NBP1-47234]

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free [NBP1-47234]

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.
CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free [NBP1-47234]

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free [NBP1-47234]

Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in Analysis (surface staining) of murine PMA, ionomycin and LPS stimulated splenocytes stained using anti-mouse CD154 (MR-1) purified antibody (concentration in sample 3 ug/ml, GAArH APC).

Applications for CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

Application
Recommended Usage

Flow Cytometry

4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:200

Immunoprecipitation

1:50
Application Notes
Functional Application: Blocking T cell costimulation in vivo. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD40 Ligand/TNFSF5

CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40 Ligand/TNFSF5 Antibody (MR-1) - BSA Free

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    • Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
      A: Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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