CD45RO Antibody (UCHL-1)

Novus Biologicals | Catalog # NBP2-29631

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Chimpanzee, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # UCHL-1
View all CD45RO Primary Antibodies »

Product Specifications

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1)

Reactivity Notes

Common Marmoset

Localization

Cell surface

Marker

T-Cell Marker

Specificity

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33104)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RO Antibody (UCHL-1)

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631]

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631]

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Staining of human tonsil
Dual RNAscope ISH-IHC: CD45RO Antibody (UCHL-1) [NBP2-29631] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD45 mRNA (ACD RNAScope probe, catalog # 601991; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-29631) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).
Immunocytochemistry/ Immunofluorescence: CD45RO Antibody (UCHL-1) [NBP2-29631]

Immunocytochemistry/ Immunofluorescence: CD45RO Antibody (UCHL-1) [NBP2-29631]

Immunocytochemistry/Immunofluorescence: CD45RO Antibody (UCHL-1) [NBP2-29631] - Immunofluorescence Analysis of human tonsil FFPE section stained with CD45RO antibody (UCHL-1)and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.
Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631]

Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631]

Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631]

Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631]

Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - CD45RO expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD45RO antibody (0.5 ug/10^6 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG, secondary antibody used to identify the positive staining on PPI negative lymphocyte gated population.

Applications for CD45RO Antibody (UCHL-1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD45RO

The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported.

Long Name

CD45R, O isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

P08575

Additional CD45RO Products

Product Documents for CD45RO Antibody (UCHL-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD45RO Antibody (UCHL-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD45RO Antibody (UCHL-1)

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  • Q: I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?

    A:

    Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at innovators@novusbio.com with any questions regarding this program.

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