CD47 Antibody (BLR131H)

Novus Biologicals | Catalog # NBP3-14713

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR131H
View all CD47 Primary Antibodies »

Product Specifications

Immunogen

residues 19-142 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD47 Antibody (BLR131H)

Western Blot: CD47 Antibody (BLR131H) [NBP3-14713]

Western Blot: CD47 Antibody (BLR131H) [NBP3-14713]

Western Blot: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000. secondary HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunocytochemistry: CD47 Antibody (BLR131H) [NBP3-14713]

Immunocytochemistry: CD47 Antibody (BLR131H) [NBP3-14713]

Immunocytochemistry: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer.
Immunohistochemistry: CD47 Antibody (BLR131H) [NBP3-14713]

Immunohistochemistry: CD47 Antibody (BLR131H) [NBP3-14713]

Immunohistochemistry: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. secondary Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000.
CD47 Antibody (BLR131H)

Simple Western: CD47 Antibody (BLR131H) [NBP3-14713] -

Simple Western: CD47 Antibody (BLR131H) [NBP3-14713] - Detection of human CD47 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD47 recombinant monoclonal antibody [BLR131H] (NBP3-14713) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.

Applications for CD47 Antibody (BLR131H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Simple Western

1:10 - 1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat [0.4 mg/mL], separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 138-142 kDa

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (BLR131H)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD47 Antibody (BLR131H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD47 Antibody (BLR131H)

Customer Reviews for CD47 Antibody (BLR131H)

There are currently no reviews for this product. Be the first to review CD47 Antibody (BLR131H) and earn rewards!

Have you used CD47 Antibody (BLR131H)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail