CD47 Antibody (CL6913) - BSA Free

Novus Biologicals | Catalog # NBP2-76511

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL6913

Format

BSA Free
View all CD47 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q08722, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD47 Antibody (CL6913) - BSA Free

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding CD47 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511] - Staining of human lung shows moderate membranous positivity in pneumocytes.
Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511]

Immunohistochemistry-Paraffin: CD47 Antibody (CL6913) [NBP2-76511] - Staining of human prostate shows strong membranous positivity in glandular cells.

Applications for CD47 Antibody (CL6913) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (CL6913) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD47 Antibody (CL6913) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail