CD47 Antibody (CL6913) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44397

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL6913

Format

Azide and BSA Free
View all CD47 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q08722, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD47 Antibody (CL6913) - Azide and BSA Free

CD47 Antibody (CL6913) - Azide and BSA Free Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44397 antibody. Corresponding CD47 RNA-seq data are presented for the same tissues.
CD47 Antibody (CL6913) - Azide and BSA Free Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Staining of human cerebral cortex shows strong positivity in neuropil.
CD47 Antibody (CL6913) - Azide and BSA Free Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Staining of human lung shows moderate membranous positivity in pneumocytes.
CD47 Antibody (CL6913) - Azide and BSA Free Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Staining of human prostate shows strong membranous positivity in glandular cells.
CD47 Antibody (CL6913) - Azide and BSA Free Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Immunohistochemistry-Paraffin: CD47 Antibody [NBP3-44397]

Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Applications for CD47 Antibody (CL6913) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (CL6913) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD47 Antibody (CL6913) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail