CD5 Antibody (rC5/6429)

Novus Biologicals | Catalog # NBP3-49022

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # rC5/6429 expressed in HEK293
View all CD5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length protein corresponding to human CD5 (Uniprot: P06127)

Localization

Cell membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for CD5 Antibody (rC5/6429)

CD5 Antibody (rC5/6429)

Western Blot: CD5 Antibody (rC5/6429) [NBP3-49022] -

Western blot analysis of Jurkat cell lysate using CD5 Antibody (rC5/6429).
CD5 Antibody (rC5/6429)

Immunohistochemistry-Paraffin: CD5 Antibody (rC5/6429) [NBP3-49022] -

Formalin-fixed, paraffin-embedded human tonsil stained with CD5 Antibody (rC5/6429).
CD5 Antibody (rC5/6429)

Immunohistochemistry-Paraffin: CD5 Antibody (rC5/6429) [NBP3-49022] -

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using CD5 Antibody (rC5/6429) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.

Applications for CD5 Antibody (rC5/6429)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD5

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (rC5/6429)

Certificate of Analysis

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Product Specific Notices for CD5 Antibody (rC5/6429)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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