CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62562

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # LU30

Format

Azide and BSA Free
View all CD55/DAF Primary Antibodies »

Product Specifications

Immunogen

Human CD55.

Specificity

The antibody binds to CD55 with a Kd of 13 nM.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free

Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free [NBP2-62562]

Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free [NBP2-62562]

Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.
Flow Cytometry: CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free [NBP2-62562]

Flow Cytometry: CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free [NBP2-62562]

Flow Cytometry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30. U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (NBP2-62562, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Applications for CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD55/DAF

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

CD55, CR, DAF, TC

Gene Symbol

CD55

Additional CD55/DAF Products

Product Documents for CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD55/DAF Antibody (LU30) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Complement Pathway

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Protocols

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