CD59 Antibody (YTH53.1) - BSA Free
Novus Biologicals | Catalog # NB100-65537
Clone YTH53.1 was used by HLDA to establish CD designation.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Functional
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # YTH53.1
Format
BSA Free
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Product Specifications
Immunogen
Human Peripheral Blood T cells
Localization
Cell membrane; lipid anchor; GPI anchor.
Specificity
NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Description
Novus Biologicals Rat CD59 Antibody (YTH53.1) - BSA Free (NB100-65537) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD59 Antibody (YTH53.1) - BSA Free
Immunohistochemistry-Paraffin: CD59 Antibody (YTH53.1) [NB100-65537] Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59
![Flow Cytometry: CD59 Antibody (YTH53.1) - BSA Free [NB100-65537]](https://resources.rndsystems.com/images/products/CD59-Antibody-YTH53-1-Flow-Cytometry-NB100-65537-img0001.jpg "Flow Cytometry: CD59 Antibody (YTH53.1) - BSA Free [NB100-65537]")
Flow Cytometry: CD59 Antibody (YTH53.1) - BSA Free [NB100-65537]
Flow Cytometry: CD59 Antibody (YTH53.1) [NB100-65537] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59 (NB100-65537).Applications for CD59 Antibody (YTH53.1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:5 - 1:10
Western Blot
1:100 - 1:2000
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD59
Additional CD59 Products
Product Documents for CD59 Antibody (YTH53.1) - BSA Free
Product Specific Notices for CD59 Antibody (YTH53.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
