CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81050

Recombinant Monoclonal Antibody. Clone 3A1E was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 3A1E

Format

Azide and BSA Free
View all CD7 Primary Antibodies »

Product Specifications

Immunogen

CD7

Specificity

Recognises human CD7.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

Western Blot: CD7 Antibody (3A1E)ChimericAzide and BSA Free [NBP2-81050]

Western Blot: CD7 Antibody (3A1E)ChimericAzide and BSA Free [NBP2-81050]

Western Blot: CD7 Antibody (3A1E) [NBP2-81050] - Western Blot using anti-CD7 antibody (3A1E) [NBP2-81050]. Jurkat cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 3A1E [NBP2-81050] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa, but this protein is glycosylated at several residues. NBP2-81050 successfully detected CD7 in Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free [NBP2-81050]

Immunocytochemistry/ Immunofluorescence: CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free [NBP2-81050]

Immunocytochemistry/Immunofluorescence: CD7 Antibody (3A1E) [NBP2-81050] - Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody (3A1E) [NBP2-81050]. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3A1E [NBP2-81050] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81050, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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