CD7 Antibody (MEM-186) - BSA Free

Novus Biologicals | Catalog # NB500-326

Clone MEM-186 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # MEM-186

Format

BSA Free
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Product Specifications

Immunogen

Human acute myelogenous leukaemia cell line KG-1.

Specificity

The MEM-186 antibody reacts with CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia. HLDA VI; WS Code T 6T-015

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for CD7 Antibody (MEM-186) - BSA Free

Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.
Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) APC.
Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - [NB500-326-Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) FITC.
Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PE-CyTM7.
CD7 Antibody (MEM-186) - BSA Free Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Flow Cytometry: CD7 Antibody (MEM-186) - BSA Free [NB500-326]

Separation of human CD7 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human CD7 (MEM-186) purified antibody (concentration in sample 0,33 ug/ml, GAM APC).

Applications for CD7 Antibody (MEM-186) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blot - Non-reducing conditions. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD7

CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.

Alternate Names

CD7, LEU-9, TP41

Entrez Gene IDs

924 (Human)

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (MEM-186) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD7 Antibody (MEM-186) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD7 Antibody (MEM-186) - BSA Free

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Protocols

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