CD8 Antibody (BLR173J)

Name: CD8 Antibody (BLR173J)
Brand: Novus Biologicals
SKU: NBP3-14746
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14746

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Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR173J

View all CD8 Primary Antibodies »

Product Specifications

Immunogen

residues 28-194 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD8 Antibody (BLR173J)

Western Blot: CD8 Antibody (BLR173J) [NBP3-14746]

Western Blot: CD8 Antibody (BLR173J) [NBP3-14746] - Rabbit anti-CD8 alpha recombinant monoclonal antibodody. Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry: CD8 Antibody (BLR173J) [NBP3-14746]

Immunohistochemistry: CD8 Antibody (BLR173J) [NBP3-14746] - Rabbit anti-CD8 alpha recombinant monoclonal antibody. Secondary HRP-conjugated goat anti-rabbit IgG (A120-501P).

Immunohistochemistry: CD8 Antibody (BLR173J) [NBP3-14746]

Immunohistochemistry: CD8 Antibody (BLR173J) [NBP3-14746] - Rabbit anti-CD8 alpha recombinant monoclonal antibody.

Immunohistochemistry: CD8 Antibody (BLR173J) [NBP3-14746] -

Immunohistochemistry: CD8 Antibody (BLR173J) [NBP3-14746] - Rabbit anti-CD8 alpha recombinantmonoclonal antibody [BLR173J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Western Blot: CD8 Antibody (BLR173J) [NBP3-14746] -

Western Blot: CD8 Antibody (BLR173J) [NBP3-14746] - Whole cell lysate (50 µg) from HEK293f and HEK293f overexpressing mouse CD8a cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-CD8-alpha recombinantmonoclonal antibody [BLR173J] used at1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F.

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Applications for CD8 Antibody (BLR173J)

Application

Recommended Usage

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

12 µl/1 mg lysate

Western Blot

1:1000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD8

CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7).

Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).

Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.

References

1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. https://doi.org/10.1146/annurev.iy.05.040187.003021

2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. https://doi.org/10.1016/B978-0-12-370607-2.00002-8.

3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. https://doi.org/10.1016/s0167-5699(00)01750-3

4. UniProt (P01732)

5. UniProt (P01731)

6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. https://doi.org/10.1016/j.immuni.2007.11.002

7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. https://doi.org/10.1016/j.coi.2004.03.015

8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. https://doi.org/10.1155/2012/189096

9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. https://doi.org/10.1007/s00281-019-00744-5

10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. https://doi.org/10.1146/annurev-med-012017-043208

Alternate Names

CD8, CD8A

Gene Symbol

CD8A

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Product Specific Notices for CD8 Antibody (BLR173J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.