CD8 Antibody (RPA-T8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80659

Clone RPA-T8 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface), In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RPA-T8

Format

Azide and BSA Free
View all CD8 Primary Antibodies »

Product Specifications

Immunogen

CD8 Antibody (RPA-T8) was developed against CD8 (CD8a; alpha chain)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD8 Antibody (RPA-T8) - Azide and BSA Free

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Cell surface flow analysis of CD8 in human PBMC using this antibody at 0.25 ug/10^6 cells. Cells were stained with primary antibody followed by a PE-conjugated goat anti-mouse secondary antibody this antibody. Green represents isotype control this antibody ; red represents anti-CD8 this antibody. Image from the standard format of this antibody.
Flow (Cell Surface): CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow (Cell Surface): CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow (Cell Surface): CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using the PE conjugate of NBP2-25195. Staining of Human peripheral blood mononuclear cells. Image from verified customer review.
Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were directly conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.
Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMC using CD8 alpha antibody. Image from verified customer review.
Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using the Allophycocyanin conjugate of NBP2-25195. Staining of CD8 in 1x106 human PBMC using 10 ul (0.1 ug) of IMG-5917G. Green represents isotype control (IMGENEX, 20107); red represents anti-CD8 antibody. Imgenex's cell surface staining flow assay kit (IMGENEX, 10084K) was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.
Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using the DyLight 405 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMCs using anti-CD8 alpha antibody. Image from verified customer review. Image from the standard format of this antibody.
Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using the FITC conjugate of NBP2-25195. Staining of CD8 in human PBMCs using 0.25 ug of (clone RPA-T8) per approximately 1x10^6 cells. Green represents isotype control this antibody, red represents anti-CD8 FITC antibody.
Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659]

Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-80659] - Analysis using the PE conjugate of NBP2-25195. Staining of CD8 in 10^6 human PBMC using 10 ul (0.1 ug) of this antibody. The shaded histogram represents human PBMC alone; green represents mouse isotype control, red represents human CD8 this antibody.

Applications for CD8 Antibody (RPA-T8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.1-0.25 ug per 1x10^6 cells

Immunohistochemistry-Frozen

reported in scientific literature (PMID 7882470)

In vitro assay

reported in multiple pieces of scientific literature

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD8

CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7).

Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).

Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.

References

1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. https://doi.org/10.1146/annurev.iy.05.040187.003021

2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. https://doi.org/10.1016/B978-0-12-370607-2.00002-8.

3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. https://doi.org/10.1016/s0167-5699(00)01750-3

4. UniProt (P01732)

5. UniProt (P01731)

6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. https://doi.org/10.1016/j.immuni.2007.11.002

7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. https://doi.org/10.1016/j.coi.2004.03.015

8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. https://doi.org/10.1155/2012/189096

9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. https://doi.org/10.1007/s00281-019-00744-5

10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. https://doi.org/10.1146/annurev-med-012017-043208

Alternate Names

CD8, CD8A

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for CD8 Antibody (RPA-T8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD8 Antibody (RPA-T8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for CD8 Antibody (RPA-T8) - Azide and BSA Free

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  • Q: What preservative-free CD8 antibodies do you offer?

    A: NBP2-34588, NBP2-34589, NBP2-34590, NBP2-34790, NBP2-54595, NBP2-80658, and NBP2-80659 are the Azide and BSA free formulations that we offer.

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