CD82/Kai-1 Antibody (C33) - BSA Free
Novus Biologicals | Catalog # NBP2-21792
![Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]](https://resources.rndsystems.com/images/products/CD82-Kai-1-Antibody-C33-Immunohistochemistry-Paraffin-NBP2-21792-img0002.jpg "Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # C33
Format
BSA Free
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Product Specifications
Immunogen
C91/PL (human HTLV-1+ T cell line).
Specificity
The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for CD82/Kai-1 Antibody (C33) - BSA Free
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).![Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]](https://resources.rndsystems.com/images/products/CD82-Kai-1-Antibody-C33-Flow-Cytometry-NBP2-21792-img0007.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]")
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.![Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]](https://resources.rndsystems.com/images/products/CD82-Kai-1-Antibody-C33-Flow-Cytometry-NBP2-21792-img0001.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]")
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.![Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]](https://resources.rndsystems.com/images/products/CD82-Kai-1-Antibody-C33-Flow-Cytometry-NBP2-21792-img0003.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]")
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Flow Cytometry: [PerCP] [NBP2-21792PCP] - A cell surface (A) and intracellular stain (B) was performed on Daudi cells with CD82/Kai-1 (C33) antibody NBP2-21792PCP along with a matched isotype control NB600-986PCP. The cell surface stain was performed in an antibody dilution of 5 ug/mL and incubated at RT for 20 minutes. Prior to intracellular staining, the cells were fixed with 4% PFA and then permeablized with 0.1 % saponin. The cells were then incubated in an antibody dilution of 5 ug/mL at RT for 30 minutes. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.![Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]](https://resources.rndsystems.com/images/products/CD82-Kai-1-Antibody-C33-Flow-Cytometry-NBP2-21792-img0005.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]")
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) FITC.![Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]](https://resources.rndsystems.com/images/products/CD82-Kai-1-Antibody-C33-Flow-Cytometry-NBP2-21792-img0006.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]")
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) biotin, streptavidin/PE.Applications for CD82/Kai-1 Antibody (C33) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1:50-1:300
Immunohistochemistry
1:50-1:300
Immunohistochemistry-Paraffin
1:50-1:300
Immunoprecipitation
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. This antibody is CyTOF ready.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD82/Kai-1
Additional CD82/Kai-1 Products
Product Documents for CD82/Kai-1 Antibody (C33) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD82/Kai-1 Antibody (C33) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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