Cdc14A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84573

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Primate - Macaca mulatta (Rhesus Macaque)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cdc14A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QQHFLEEKQASLWVQGDIFRSKLKNRPSSEGSINKILSGLDDMSIGGNLSKTQNMERFGEDNLEDDDVEMKNGITQGDKLRAL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%). Monkey reactivity reported in scientific literature (PMID: 29293958).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdc14A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cdc14A Antibody [NBP1-84573]

Immunocytochemistry/ Immunofluorescence: Cdc14A Antibody [NBP1-84573]

Immunocytochemistry/Immunofluorescence: Cdc14A Antibody [NBP1-84573] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.
Cdc14A Antibody - BSA Free Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Cdc14A Antibody - BSA Free Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Staining of human lymph node shows moderate nuclear positivity in non-germinal center cells.
Cdc14A Antibody - BSA Free Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Staining of human duodenum shows moderate nuclear positivity in glandular cells.
Cdc14A Antibody - BSA Free Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Immunohistochemistry-Paraffin: Cdc14A Antibody - BSA Free [NBP1-84573]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for Cdc14A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cdc14A

Overproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53.

Alternate Names

CDC10 (cell division cycle 10, S. cerevisiae, homolog), cdc14, CDC14 cell division cycle 14 homolog A, CDC14 cell division cycle 14 homolog A (S. cerevisiae), Cdc14A1, Cdc14A2, dual specificity protein phosphatase CDC14A, EC 3.1.3.16, EC 3.1.3.48, hCDC14

Gene Symbol

CDC14A

Additional Cdc14A Products

Product Documents for Cdc14A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cdc14A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cdc14A Antibody - BSA Free

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Protocols

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