CDC5L Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85720

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC5L Antibody - BSA Free

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720] - Staining of human cerebral cortex shows strong nuclear positivity in neurons and glial cells.
Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720]

Immunohistochemistry-Paraffin: CDC5L Antibody [NBP1-85720] - Staining of human rectum shows moderate to strong nuclear positivity in glandular cells.
CDC5L Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: CDC5L Antibody - BSA Free [NBP1-85720]

Immunocytochemistry/ Immunofluorescence: CDC5L Antibody - BSA Free [NBP1-85720]

Staining of human cell line U-2 OS shows localization to nucleoplasm.
CDC5L Antibody - BSA Free Western Blot: CDC5L Antibody - BSA Free [NBP1-85720]

Western Blot: CDC5L Antibody - BSA Free [NBP1-85720]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4

Applications for CDC5L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC5L

CDC5L is encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq]

Alternate Names

CDC5, CDC5 (cell division cycle 5, S. pombe, homolog)-like, CDC5 cell division cycle 5-like (S. pombe), Cdc5-like protein, Cdc5-related protein, CEF1, cell division cycle 5-like protein, dJ319D22.1, dJ319D22.1 (CDC5-like protein), hCDC5, KIAA0432, PCDC5RPCDC5-LIKE, Pombe cdc5-related protein

Gene Symbol

CDC5L

Additional CDC5L Products

Product Documents for CDC5L Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CDC5L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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