CDCA5 Antibody (1Y1C9)

Novus Biologicals | Catalog # NBP3-16318

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1Y1C9 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 153-252 of human CDCA5 (Q96FF9). GSASTSTPGRRSCFGFEGLLGAEDLSGVSPVVCSKLTEVPRVCAKPWAPDMTLPGISPPPEKQKRKKKKMPEILKTELDEWAAAMNAEFEAAEQFDLLVE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDCA5 Antibody (1Y1C9)

Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318]

Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318]

Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: CDCA5 Antibody (1Y1C9) [NBP3-16318]

Immunohistochemistry-Paraffin: CDCA5 Antibody (1Y1C9) [NBP3-16318]

Immunohistochemistry-Paraffin: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Immunohistochemistry of paraffin-embedded human thyroid cancer using CDCA5 Rabbit mAb (NBP3-16318) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for CDCA5 Antibody (1Y1C9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDCA5

Regulator of sister chromatid cohesion in mitosis. May act by regulating the ability of the cohesin complexto mediate sister chromatid cohesion, perhaps by altering the nature of the interaction of cohesin with thechromosomes

Alternate Names

cell division cycle associated 5, Cell division cycle-associated protein 5, MGC16386, p35, SORORIN

Gene Symbol

CDCA5

Additional CDCA5 Products

Product Documents for CDCA5 Antibody (1Y1C9)

Certificate of Analysis

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Product Specific Notices for CDCA5 Antibody (1Y1C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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