Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2D9

Format

Azide and BSA Free
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDK2 Antibody (OTI2D9) - Azide and BSA Free

Western Blot: CDK2 Antibody (OTI2D9)Azide and BSA Free [NBP2-70392]

Western Blot: CDK2 Antibody (OTI2D9)Azide and BSA Free [NBP2-70392]

Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.
Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.
Immunohistochemistry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Immunohistochemistry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Immunohistochemistry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.
Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cdk2 antibody, and then analyzed by flow cytometry.
Western Blot: CDK2 Antibody (OTI2D9)Azide and BSA Free [NBP2-70392]

Western Blot: CDK2 Antibody (OTI2D9)Azide and BSA Free [NBP2-70392]

Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.
Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392]

Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for CDK2 Antibody (OTI2D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 Antibody (OTI2D9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDK2 Antibody (OTI2D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail