CDK2 [p Thr160] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05409

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around T160 of human CDK2 (NP_001789.2?/NP_001249.1). TYTHE

Modification

p Thr160

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CDK2 [p Thr160] Antibody - BSA Free (NBP3-05409) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CDK2 [p Thr160] Antibody - BSA Free

Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody - Azide and BSA Free [NBP3-05409]

Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody - Azide and BSA Free [NBP3-05409]

Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).
CDK2 [p Thr160] Antibody

Western Blot: CDK2 [p Thr160] Antibody [NBP3-05409] -

Western Blot: CDK2 [p Thr160] Antibody [NBP3-05409] - analysis of C6, using Phospho-CDK2-T160 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
CDK2 [p Thr160] Antibody - Azide and BSA Free

Western Blot: CDK2 [p Thr160] Antibody - Azide and BSA Free [CDK2] -

Western Blot: CDK2 [p Thr160] Antibody - Azide and BSA Free [CDK2] - Western blot analysis of lysates from C6 cells using CDK2 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 20 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Applications for CDK2 [p Thr160] Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 [p Thr160] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CDK2 [p Thr160] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail