CDK4 Antibody (CL8102) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44220

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL8102

Format

Azide and BSA Free
View all CDK4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P11802, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CDK4 Antibody (CL8102) - Azide and BSA Free

CDK4 Antibody (CL8102) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Staining of human endometrium shows strong nuclear positivity in cells in endometrial stroma.
CDK4 Antibody (CL8102) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Staining of human liver cancer shows strong nuclear positivity in tumor cells.
CDK4 Antibody (CL8102) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
CDK4 Antibody (CL8102) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Staining of human prostate shows strong nuclear positivity in smooth muscle cells.
CDK4 Antibody (CL8102) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Immunohistochemistry-Paraffin: CDK4 Antibody [NBP3-44220]

Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
CDK4 Antibody (CL8102) - Azide and BSA Free Western Blot: CDK4 Antibody [NBP3-44220]

Western Blot: CDK4 Antibody [NBP3-44220]

Analysis in human cell line RH-30.
CDK4 Antibody (CL8102) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: CDK4 Antibody [NBP3-44220]

Immunocytochemistry/ Immunofluorescence: CDK4 Antibody [NBP3-44220]

Staining of RH-30 cells using the Anti-CDK4 monoclonal antibody).

Applications for CDK4 Antibody (CL8102) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDK4

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Gene Symbol

CDK4

Additional CDK4 Products

Product Documents for CDK4 Antibody (CL8102) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CDK4 Antibody (CL8102) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

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