CEACAM8/CD66b Antibody (BLR111H)

Novus Biologicals | Catalog # NBP3-14700

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR111H
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Product Specifications

Immunogen

residues 23-320 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CEACAM8/CD66b Antibody (BLR111H)

Western Blot: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Western Blot: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Western Blot: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Whole cell lysate (10 ug) from Raji, K-562, HL-60, THP-1, KG-1, MUTZ-3, SR, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit antiCEACAM8/CD66b recombinant monoclonal antibody [BLR117H] used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds
Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody[BLR111H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody [BLR111H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Immunoprecipitation: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]

Immunoprecipitation: CEACAM8/CD66b Antibody [NBP3-14700] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HL-60 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiCEACAM8/CD66b recombinant monoclonal antibody[BLR117H] (A700-111 lot 1) used at 20 ul/mg lysate. Forblotting immunoprecipitated CEACAM8/CD66b, A700-111was used at 1:1000. Chemiluminescence with an exposuretime of 30 seconds.

Applications for CEACAM8/CD66b Antibody (BLR111H)

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunoprecipitation

20ul/1mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CEACAM-8/CD66b

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Gene Symbol

CEACAM8

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (BLR111H)

Certificate of Analysis

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Product Specific Notices for CEACAM8/CD66b Antibody (BLR111H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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