CEACAM8/CD66b Antibody (BLR111H)
Novus Biologicals | Catalog # NBP3-14700
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR111H
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Product Specifications
Immunogen
residues 23-320 (ECD)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for CEACAM8/CD66b Antibody (BLR111H)
Western Blot: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]
Western Blot: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Whole cell lysate (10 ug) from Raji, K-562, HL-60, THP-1, KG-1, MUTZ-3, SR, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit antiCEACAM8/CD66b recombinant monoclonal antibody [BLR117H] used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 secondsImmunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]
Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody[BLR111H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]
Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody [BLR111H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.Immunoprecipitation: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700]
Immunoprecipitation: CEACAM8/CD66b Antibody [NBP3-14700] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HL-60 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiCEACAM8/CD66b recombinant monoclonal antibody[BLR117H] (A700-111 lot 1) used at 20 ul/mg lysate. Forblotting immunoprecipitated CEACAM8/CD66b, A700-111was used at 1:1000. Chemiluminescence with an exposuretime of 30 seconds.Applications for CEACAM8/CD66b Antibody (BLR111H)
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunoprecipitation
20ul/1mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: CEACAM-8/CD66b
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Alternate Names
CD66b, CD67, CEACAM8, CGM6, NCA-95
Gene Symbol
CEACAM8
Additional CEACAM-8/CD66b Products
Product Documents for CEACAM8/CD66b Antibody (BLR111H)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CEACAM8/CD66b Antibody (BLR111H)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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