CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80664

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # G10F5

Format

Azide and BSA Free
View all CEACAM-8/CD66b Primary Antibodies »

Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Predicted to cross react with Chimpanzee.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific wi
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody. Image from the standard format of this antibody.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Image from verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Image from verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Image from verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC Image from the standard format of this antibody.

Applications for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ug/million cells

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Gene Symbol

CEACAM8

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

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Protocols

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