CEACAM8/CD66b Antibody (G10F5) - BSA Free
Novus Biologicals | Catalog # NB100-77808
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # G10F5
Format
BSA Free
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Product Specifications
Immunogen
Human peripheral blood cells.
Reactivity Notes
Predicited to cross react with Chimpanzee. Use in Mouse reported in scientific literature (PMID:33122293).
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Scientific Data Images for CEACAM8/CD66b Antibody (G10F5) - BSA Free
Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.Applications for CEACAM8/CD66b Antibody (G10F5) - BSA Free
Application
Recommended Usage
Flow Cytometry
1ug/million cells
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 33122293)
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Application Notes
It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.
Reviewed Applications
Read 2 reviews rated 5 using NB100-77808 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
IgM purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CEACAM-8/CD66b
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Alternate Names
CD66b, CD67, CEACAM8, CGM6, NCA-95, cd66b clone g10f5, cd66b G10F5, cd66b granulocyte marker, cd66b ihc, cd66b marker, cd66b neutrophil marker, cd66b neutrophils
Entrez Gene IDs
1088 (Human)
Gene Symbol
CEACAM8
UniProt
Additional CEACAM-8/CD66b Products
Product Documents for CEACAM8/CD66b Antibody (G10F5) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CEACAM8/CD66b Antibody (G10F5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CEACAM8/CD66b Antibody (G10F5) - BSA Free
Customer Reviews for CEACAM8/CD66b Antibody (G10F5) - BSA Free (2)
5 out of 5
2 Customer Ratings
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Customer Images
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Application: Immunohistochemistry-ParaffinSample Tested: Human SpleenSpecies: HumanVerified Customer | Posted 07/28/2020granulocytes were positive for CD66BIHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS
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Application: ImmunohistochemistrySample Tested: Liver cancer tissueSpecies: HumanVerified Customer | Posted 06/09/2017Staining of CD66b on FFPE section of human liver. 1ry Ab is NB100-77808. 2ry Ab is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei.IHC - Single staining of CEACAM-8/CD66b on FFPE section of human liver. Primary Ab is mouse IgM kappa monoclonal Ab from Novus Biologicals (NB100-77808). Secondary Ab is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. The cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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