CEACAM8/CD66b Antibody (G10F5) - BSA Free

Novus Biologicals | Catalog # NB100-77808

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # G10F5

Format

BSA Free
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Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Predicited to cross react with Chimpanzee. Use in Mouse reported in scientific literature (PMID:33122293).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for CEACAM8/CD66b Antibody (G10F5) - BSA Free

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.
Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.
Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808]

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.

Applications for CEACAM8/CD66b Antibody (G10F5) - BSA Free

Application
Recommended Usage

Flow Cytometry

1ug/million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 33122293)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 5 using NB100-77808 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95, cd66b clone g10f5, cd66b G10F5, cd66b granulocyte marker, cd66b ihc, cd66b marker, cd66b neutrophil marker, cd66b neutrophils

Entrez Gene IDs

1088 (Human)

Gene Symbol

CEACAM8

UniProt

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (G10F5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CEACAM8/CD66b Antibody (G10F5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CEACAM8/CD66b Antibody (G10F5) - BSA Free

Customer Reviews for CEACAM8/CD66b Antibody (G10F5) - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
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1 Stars
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Customer Images


Showing  1 - 2 of 2 reviews Showing All
Filter By:
  • CEACAM8/CD66b Antibody (G10F5)
    Name: Masahiro Hirata
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Spleen
    Species: Human
    Verified Customer | Posted 07/28/2020
    granulocytes were positive for CD66B
    IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS
    CEACAM8/CD66b Antibody (G10F5) - BSA Free NB100-77808
  • CEACAM8/CD66b Antibody (G10F5)
    Name: Manuel Flores
    Application: Immunohistochemistry
    Sample Tested: Liver cancer tissue
    Species: Human
    Verified Customer | Posted 06/09/2017
    Staining of CD66b on FFPE section of human liver. 1ry Ab is NB100-77808. 2ry Ab is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei.
    IHC - Single staining of CEACAM-8/CD66b on FFPE section of human liver. Primary Ab is mouse IgM kappa monoclonal Ab from Novus Biologicals (NB100-77808). Secondary Ab is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. The cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal.

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Showing  1 - 2 of 2 reviews Showing All

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