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TACS XTT Cell Proliferation Assay

Catalog #: 4891-025-K
27 Citations 2 Reviews Datasheet / COA / SDS
A sensitive kit for the measurement of cell proliferation using XTT
Catalog # Availability Size / Price Qty
4891-025-K
TACS XTT Cell Proliferation/Viability Assay_4891-025-K
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Product Details
Citations (27)
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Reviews (2)
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TACS XTT Cell Proliferation Assay Summary

Provides a sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide.
 

Key Benefits

• Allows measurement of cell proliferation, cell viability or cytotoxicity.
• Rapid determination in microplates (no solubilization step as in an MTT assay).
• Reproducible and sensitive results.
• No radioactivity.
• Ideal for high throughput assays (no washing or other steps that can cause cell loss and variability).

Why Use the TACS XTT Cell Proliferation Assay?

The XTT Cell Proliferation Assay (XTT-CPA) is a sensitive kit for the measurement of cell proliferation. Cleavage of the tetrazolium salt to formazan occurs via the succinate-tetrazolium reductase system in the mitochondria of metabolically active cells.

Kit Contents

• XTT Reagent
• XTT Activator

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Scientific Data

N/A TACS XTT Cell Proliferation/Viability Assay_4891-025-K View Larger

Relative Cell Number Measured using the TACS XTT Cell Proliferation/Viability Assay. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densities. Cells were incubated with XTT for 6 hours at 27 °C and absorbance was read using an ELISA plate reader.

Citations for TACS XTT Cell Proliferation Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species-p90RSK-Vascular Endothelial Growth Factor Receptor 3 Pathway
    Authors: P Banerjee, S Kumaravel, S Roy, N Gaddam, J Odeh, KJ Bayless, S Glaser, S Chakrabort
    Cells, 2023;12(4):.  2023
  2. Evaluation of the antioxidant potency of Greek honey from the Taygetos and Pindos mountains using a combination of cellular and molecular methods
    Authors: A Patouna, P Vardakas, Z Skaperda, DA Spandidos, D Kouretas
    Molecular Medicine Reports, 2023;27(2):.  2023
  3. Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling
    Authors: P Melo, G Montalbano, E Boggio, CL Gigliotti, C Dianzani, U Dianzani, C Vitale-Bro, S Fiorilli
    Polymers, 2022;14(18):.  2022
  4. The long noncoding RNA H19 regulates tumor plasticity in neuroendocrine prostate cancer
    Authors: N Singh, VR Ramnarine, JH Song, R Pandey, SKR Padi, M Nouri, V Olive, M Kobelev, K Okumura, D McCarthy, MM Hanna, P Mukherjee, B Sun, BR Lee, JB Parker, D Chakravart, NA Warfel, M Zhou, JJ Bearss, EA Gibb, M Alshalalfa, RJ Karnes, EJ Small, R Aggarwal, F Feng, Y Wang, R Buttyan, A Zoubeidi, M Rubin, M Gleave, FJ Slack, E Davicioni, H Beltran, C Collins, AS Kraft
    Nature Communications, 2021;12(1):7349.  2021
  5. Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury
    Authors: D Medica, R Franzin, A Stasi, G Castellano, M Migliori, V Panichi, F Figliolini, L Gesualdo, G Camussi, V Cantaluppi
    Cells, 2021;10(7):.  2021
  6. Anti-inflammatory activity of Nectandra angustifolia (Laurel Amarillo) ethanolic extract
    Authors: L Ferrini, JP Rodríguez, JP Melana Col, G Olea, GA Ojeda, G Ricciardi, AM Torres, MV Aguirre
    Journal of ethnopharmacology, 2021;272(0):113937.  2021
  7. FAM83G Is a Novel Inducer of Apoptosis
    Authors: J Okada, N Sunaga, E Yamada, T Saito, A Ozawa, Y Nakajima, K Okada, JE Pessin, S Okada, M Yamada
    Molecules, 2020;25(12):.  2020
  8. Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells
    Authors: CH Yeh, M Bellon, F Wang, H Zhang, L Fu, C Nicot
    Mol. Cancer, 2020;19(1):139.  2020
  9. Transient receptor potential ion channel TRPM2 promotes AML proliferation and survival through modulation of mitochondrial function, ROS, and autophagy
    Authors: SJ Chen, L Bao, K Keefer, S Shanmughap, L Chen, J Lee, J Wang, XQ Zhang, I Hirschler-, S Merali, C Merali, Y Imamura, S Dovat, M Madesh, JY Cheung, HG Wang, BA Miller
    Cell Death Dis, 2020;11(4):247.  2020
  10. Proanthocyanidins from the stem bark of Rhus tripartita ameliorate methylgloxal-induced endothelial cell apoptosis
    Authors: AS Alqahtani, WM Abdel-Mage, AA Shahat, MK Parvez, MS Al-Dosari, A Malik, MS Abdel-Kade, MS Alsaid
    J Food Drug Anal, 2019;27(3):758-765.  2019
  11. The BET inhibitor GS-5829 targets chronic lymphocytic leukemia cells and their supportive microenvironment
    Authors: E Kim, E Ten Hacken, M Sivina, A Clarke, PA Thompson, N Jain, A Ferrajoli, Z Estrov, MJ Keating, WG Wierda, KN Bhalla, JA Burger
    Leukemia, 2019;0(0):.  2019
  12. The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2
    Authors: L Bao, F Festa, CS Freet, JP Lee, IM Hirschler-, SJ Chen, KA Keefer, HG Wang, AD Patterson, JY Cheung, BA Miller
    Sci Rep, 2019;9(1):14132.  2019
  13. Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells
    Authors: HM Tunset, AJ Feuerherm, LM Selvik, B Johansen, SA Moestue
    Int J Mol Sci, 2019;20(19):.  2019
  14. Hepatoprotective effect of Solanum surattense leaf extract against chemical- induced oxidative and apoptotic injury in rats
    Authors: MK Parvez, MS Al-Dosari, AH Arbab, P Alam, MS Alsaid, AA Khan
    BMC Complement Altern Med, 2019;19(1):154.  2019
  15. Potent and selective EGFR inhibitors based on 5-aryl-7H-pyrrolopyrimidin-4-amines
    Authors: AC Reiersølmo, TI Aarhus, S Eckelt, KG Nørsett, E Sundby, BH Hoff
    Bioorg. Chem., 2019;88(0):102918.  2019
  16. Drug Repositioning Inferred from E2F1-Coregulator Interactions Studies for the Prevention and Treatment of Metastatic Cancers
    Authors: D Goody, SK Gupta, D Engelmann, A Spitschak, S Marquardt, S Mikkat, C Meier, C Hauser, JP Gundlach, JH Egberts, H Martin, T Schumacher, A Trauzold, O Wolkenhaue, S Logotheti, BM Pützer
    Theranostics, 2019;9(5):1490-1509.  2019
  17. Intraocular DHODH-inhibitor PP-001 suppresses relapsing experimental uveitis and cytokine production of human lymphocytes, but not of RPE cells
    Authors: M Diedrichs-, S Niesik, CS Priglinger, SR Thurau, F Obermayr, S Sperl, G Wildner
    J Neuroinflammation, 2018;15(1):54.  2018
  18. Stabilization of telomere G-quadruplexes interferes with human herpesvirus 6A chromosomal integration
    Authors: S Gilbert-Gi, A Gravel, S Artusi, SN Richter, N Wallaschek, BB Kaufer, L Flamand
    J. Virol., 2017;0(0):.  2017
  19. Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells.
    Authors: Hagelgans A, Nacke B, Zamaraeva M, Siegert G, Menschikowski M
    Anticancer Res, 2014;34(4):1723-9.  2014
  20. Nucleobindin-2 is a positive modulator of EGF-dependent signals leading to enhancement of cell growth and suppression of adipocyte differentiation.
    Authors: Tagaya Y, Miura A, Okada S, Ohshima K, Mori M
    Endocrinology, 0;153(7):3308-19.  0
  21. Resetting microbiota by Lactobacillus reuteri inhibits T reg deficiency-induced autoimmunity via adenosine A2A receptors.
    Authors: He B, Hoang T, Wang T, Ferris M, Taylor C, Tian X, Luo M, Tran D, Zhou J, Tatevian N, Luo F, Molina J, Blackburn M, Gomez T, Roos S, Rhoads J, Liu Y
    J Exp Med, 0;214(1):107-123.  0
  22. Anti-PD-L1 Efficacy Can Be Enhanced by Inhibition of Myeloid-Derived Suppressor Cells with a Selective Inhibitor of PI3Kdelta/gamma.
    Authors: Davis R, Moore E, Clavijo P, Friedman J, Cash H, Chen Z, Silvin C, Van Waes C, Allen C
    Cancer Res, 0;77(10):2607-2619.  0
  23. Depletion of the Human Ion Channel TRPM2 in Neuroblastoma Demonstrates Its Key Role in Cell Survival through Modulation of Mitochondrial Reactive Oxygen Species and Bioenergetics.
    Authors: Bao L, Chen S, Conrad K, Keefer K, Abraham T, Lee J, Wang J, Zhang X, Hirschler-Laszkiewicz I, Wang H, Dovat S, Gans B, Madesh M, Cheung J, Miller B
    J Biol Chem, 0;291(47):24449-24464.  0
  24. Methotraxate-Loaded Hybrid Nanoconstructs Target Vascular Lesions and Inhibit Atherosclerosis Progression in ApoE<sup>-/-</sup> Mice.
    Authors: Stigliano C, Ramirez M, Singh J, Aryal S, Key J, Blanco E, Decuzzi P
    Adv Healthc Mater, 0;6(13):.  0
  25. Blockade of CCR5 receptor prevents M2 microglia phenotype in a microglia-glioma paradigm.
    Authors: Laudati E, Curro D, Navarra P, Lisi L
    Neurochem Int, 0;108(0):100-108.  0
  26. A splice variant of the human ion channel TRPM2 modulates neuroblastoma tumor growth through hypoxia-inducible factor (HIF)-1/2alpha.
    Authors: Chen S, Hoffman N, Shanmughapriya S, Bao L, Keefer K, Conrad K, Merali S, Takahashi Y, Abraham T, Hirschler-Laszkiewicz I, Wang J, Zhang X, Song J, Barrero C, Shi Y, Kawasawa Y, Bayerl M, Sun T, Barbour M, Wang H, Madesh M, Cheung J, Miller B
    J Biol Chem, 0;289(52):36284-302.  0
  27. Akt-activated endothelium constitutes the niche for residual disease and resistance to bevacizumab in ovarian cancer.
    Authors: Guerrouahen B, Pasquier J, Kaoud N, Maleki M, Beauchamp M, Yasmeen A, Ghiabi P, Lis R, Vidal F, Saleh A, Gotlieb W, Rafii S, Rafii A
    Mol Cancer Ther, 0;13(12):3123-36.  0

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TACS XTT Cell Proliferation Assay
By Lucia Lisi on 05/19/2021
TACS XTT Cell Proliferation Assay 4891-025-K
TACS XTT Cell Proliferation Assay
By Leandro Ferrini on 03/25/2021

RAW 264.7 cell line cultured with DMEM + fetal bovine serum 10%. Different cell quantities were seeded in 96 well plates (each quantity per triplicate). Incubation with XTT solution was 4 h. Data shows average + SEM.

TACS XTT Cell Proliferation Assay 4891-025-K