TACS XTT Cell Proliferation Assay

R&D Systems | Catalog # 4891-025-K

A sensitive kit for the measurement of cell proliferation using XTT
R&D Systems
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Key Product Details

Features

Provides a sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide.

Key Benefits

  • Allows measurement of cell proliferation, cell viability or cytotoxicity.
  • Rapid determination in microplates (no solubilization step as in an MTT assay).
  • Reproducible and sensitive results.
  • No radioactivity.
  • Ideal for high throughput assays (no washing or other steps that can cause cell loss and variability).

Product Summary for TACS XTT Cell Proliferation Assay

Why Use the TACS XTT Cell Proliferation Assay?
The XTT Cell Proliferation Assay (XTT-CPA) is a sensitive kit for the measurement of cell proliferation. Cleavage of the tetrazolium salt to formazan occurs via the succinate-tetrazolium reductase system in the mitochondria of metabolically active cells.

Scientific Data Images for TACS XTT Cell Proliferation Assay

TACS XTT Cell Proliferation/Viability Assay_4891-025-K

Relative Cell Number Measured using the TACS XTT Cell Proliferation/Viability Assay.

Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densities. Cells were incubated with XTT for 6 hours at 27 °C and absorbance was read using an ELISA plate reader.

Kit Contents for TACS XTT Cell Proliferation Assay

  • XTT Reagent
  • XTT Activator

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for TACS XTT Cell Proliferation Assay

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Related Research Areas

Cell Cycle

Citations for TACS XTT Cell Proliferation Assay

Customer Reviews for TACS XTT Cell Proliferation Assay (3)

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  • TACS XTT Cell Proliferation Assay
    Name: Anonymous
    Verified Customer | Posted 08/29/2024
    TACS XTT Cell Proliferation Assay 4891-025-K
  • TACS XTT Cell Proliferation Assay
    Name: Lucia Lisi
    Verified Customer | Posted 05/19/2021
    TACS XTT Cell Proliferation Assay 4891-025-K
  • TACS XTT Cell Proliferation Assay
    Name: Leandro Ferrini
    Verified Customer | Posted 03/25/2021
    RAW 264.7 cell line cultured with DMEM + fetal bovine serum 10%. Different cell quantities were seeded in 96 well plates (each quantity per triplicate). Incubation with XTT solution was 4 h. Data shows average + SEM.
    TACS XTT Cell Proliferation Assay 4891-025-K

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