Proteome Profiler Human Apoptosis Array Kit
Proteome Profiler Human Apoptosis Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of human apoptosis-related proteins. Validated for analyte detection in cell lysates.
Key Benefits
- Detects 35 human apoptosis-related proteins simultaneously
- Requires no specialized equipment
Principle of the Assay
The Proteome Profiler Human Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.
Why Use an Antibody Array to Detect Apoptosis-Related Proteins?
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the relative levels of these apoptosis-related proteins in a single sample. | ||
Bad | TRAIL R1/DR4 | PON2 |
Bax | TRAIL R2/DR5 | p21/CIP1/CDNK1A |
Bcl-2 | FADD | p27/Kip1 |
Bcl-x | Fas/TNFSF6 | Phospho-p53 (S15) |
Pro-Caspase-3 | HIF-1 alpha | Phospho-p53 (S46) |
Cleaved Caspase-3 | HO-1/HMOX1/HSP32 | Phospho-p53 (S392) |
Catalase | HO-2/HMOX2 | Phospho-Rad17 (S635) |
cIAP-1 | HSP27 | Pro-Caspase-3 |
cIAP-2 | HSP60 | SMAC/Diablo |
Claspin | HSP70 | Survivin |
Clusterin | HTRA2/Omi | TNF RI/TNFRSF1A |
Cytochrome c | Livin | XIAP |
Assays for analytes represented in the Human Apoptosis Array Kit
Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development Systems | DuoSet® IC ELISA Development Systems (Total) | DuoSet® IC ELISA Development Systems (Phospho or Active) |
Bad | DYC819 | |||
Bax | DYC820 | |||
Bcl-2 | DYC827B | |||
Bcl-x | ||||
Pro-Caspase-3 | ||||
Cleaved Caspase-3 | KM300 | DYC835 | ||
Catalase | ||||
cIAP-1 | ||||
cIAP-2 | ||||
Claspin | ||||
Clusterin | DCLU00 | DY5874 | DYC2937 | |
Cytochrome c | DCTC0 | DYC897 | ||
TRAIL R1/DR4 | ||||
TRAIL R2/DR5 | ||||
FADD | ||||
Fas/TNFSF6 | DFS00 | DY326 | ||
HIF-1alpha | DYC1935 | |||
HO-1/HMOX1/HSP32 | DYC3776 | |||
HO-2/HMOX2 | ||||
HSP27 | DYC1580 | DYC2314 | ||
HSP60 | DYC1800 | |||
HSP70 | DYC1663 | |||
HTRA2/Omi | ||||
Livin | ||||
PON2 | DYC4344 | |||
p21/CIP1/CDNK1A | DYC1047 | |||
p27/Kip1 | DYC2256 | |||
phospho-p53 (S15) | DYC1043 | DYC1839 | ||
phospho-p53 (S46) | DYC1043 | DYC1489 | ||
phospho-p53 (S392) | DYC1043 | DYC2996 | ||
phospho-Rad17 (S635) | ||||
SMAC/Diablo | DYC789 | |||
Survivin | DSV00 | DYC647 | ||
TNF RI/TNFRSF1A | DRT100 | DY225 | ||
XIAP | DYC822 |
Western blotting antibodies for analytes related to the Human Apoptosis Array Kit
Analyte | Phospho-Specific Antibodies | Affinity Purified Polyclonal Antibodies (Total) | Monoclonal Antibodies (Total) | Labeled Antibodies (Total) |
Bad | AF819 | MAB6405 | ||
Bax | AF820 | MAB846, MAB883, 2282-MC-100 | ||
Bcl-2 | AF810 | MAB8272 | ||
Bcl-x | AF800 | |||
Cleaved Caspase-3 | MAB835 | |||
Catalase | AF3398 | |||
cIAP-1 | AF8181 | MAB818 | ||
cIAP-2 | AF8171 | MAB817, MAB3400, MAB3310 | BAF8171 | |
Claspin | AF3310 | BAF3310 | ||
Clusterin | AF2937 | MAB2937 | BAF2937 | |
Cytochrome c | MAB897 | |||
Trail R1/DR4 | AF347 | BAF347 | ||
Trail R2/DR5 | AF631 | BAF631 | ||
FADD | MAB7047 (S194) | AF2938 | ||
Fas/TNFSF6 | AF326 | MAB142 | BAF326 | |
HIF-1alpha | AF1935 | MAB1536 | ||
HO-1/HMOX1/ HSP32 | AF3776 | MAB3776 | ||
HO-2/HMOX2 | AF3170 | MAB3170 | ||
HSP27 | AF2314 (S78/S82) | AF1580, AF15801 | ||
HSP60 | AF1800 | MAB1800 | ||
HSP70 | AF1663 | MAB1663 | ||
HTRA2/Omi | AF1458 | MAB1458 | ||
Livin | AF1161 | |||
PON2 | AF4344 | MAB4344 | ||
p21/CIP1/CDNK1A | AF1047 | MAB1047 | BAF1047 | |
p27/Kip1 | AF1555 (T157), AF3994 (T198) | AF2256 | MAB2256 | |
phospho-p53 (S15) | AF1043 (S15), MAB1839 (S15) | AF1355 | MAB1355 | BAF1355, HAF1355 |
phospho-p53 (S46) | AF1489 (S46), MAB1489 (S46) | AF1355 | MAB1355 | BAF1355, HAF1355 |
phospho-p53 (S392) | AF2996 (S392) | AF1355 | MAB1355 | BAF1355, HAF1355 |
phospho-Rad17 (S635) | AF1374 (S635) | AF1926 | ||
SMAC/Diablo | AF789, AF7891 | BAF789 | ||
TNF RI/TNFRSF1A | AF225 | MAB225, MAB625 | BAF225 | |
XIAP | AF8221 | MAB822 | BAF8221 |
R&D Systems' Apoptosis Array Kit is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of expression of many apoptosis-related proteins in a single sample. No specialized equipment is necessary.
Specifications
Product Datasheets
Data Example
Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit.
Figure 1A: K562 human chronic myelogenous leukemia cells.
Figure 1B: THP-1 human acute monocytic leukemia cells.
Figure 1C: SK-Mel-28 human malignant melanoma cells.
Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. Jurkat human acute T cell leukemia cells were either untreated or treated with 25 µM etoposide for 6 hours. Etoposide is a topoisomerase II inhibitor that causes caspase-3 cleavage as apoptosis is induced.
Citations for Proteome Profiler Human Apoptosis Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Chemical, Physical and Biological Triggers of Evolutionary Conserved Bcl-xL-Mediated Apoptosis
Authors: A Ianevski, E Kulesskiy, K Krpina, G Lou, Y Aman, A Bugai, K Aasumets, Y Akimov, D Bulanova, K Gildemann, AF Arutyunyan, OY Susova, AL Zhuze, P Ji, W Wang, T Holien, M Bugge, E Zusinaite, V Oksenych, H Lysvand, JM Gerhold, M Bjørås, P Johansen, A Waage, CA Heckman, EF Fang, DE Kainov
Cancers (Basel), 2020;12(6):. 2020
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Robustaflavone induces G0/G1 cell cycle arrest and apoptosis in human umbilical vein endothelial cells and exhibits anti-angiogenic effects in vivo
Authors: WK Sim, JH Park, KY Kim, IS Chung
Sci Rep, 2020;10(1):11070. 2020
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The TUSC2 Tumour Suppressor Inhibits the Malignant Phenotype of Human Thyroid Cancer Cells via SMAC/DIABLO Protein
Authors: RM Mariniello, FM Orlandella, AE Stefano, PLC Iervolino, G Smaldone, N Luciano, N Cervone, F Munciguerr, S Esposito, P Mirabelli, G Salvatore
Int J Mol Sci, 2020;21(3):. 2020
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Redox Modulation and Induction of Ferroptosis as a New Therapeutic Strategy in Hepatocellular Carcinoma
Authors: J Lippmann, K Petri, S Fulda, J Liese
Transl Oncol, 2020;13(8):100785. 2020
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Identification of UAP1L1 as tumor promotor in gastric cancer through regulation of CDK6
Authors: J Qi, S Liu, W Liu, G Cai, G Liao
Aging (Albany NY), 2020;12(8):6904-6927. 2020
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Apoptosis Induced by (-)-Epicatechin in Human Breast Cancer Cells is Mediated by Reactive Oxygen Species
Authors: F Pereyra-Ve, IM Olivares-C, AG Perez-Ruiz, JP Luna-Arias, JR García-Sán
Molecules, 2020;25(5):. 2020
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Aging-regulated anti-apoptotic long non-coding RNA Sarrah augments recovery from acute myocardial infarction
Authors: DJ Trembinski, DI Bink, K Theodorou, J Sommer, A Fischer, A van Bergen, CC Kuo, IG Costa, C Schürmann, MS Leisegang, RP Brandes, T Alekseeva, B Brill, A Wietelmann, CN Johnson, A Spring-Con, M Kaulich, S Werfel, S Engelhardt, MN Hirt, K Yorgan, T Eschenhage, L Kirchhof, P Hofmann, N Jaé, I Wittig, N Hamdani, C Bischof, J Krishnan, RH Houtkooper, S Dimmeler, RA Boon
Nat Commun, 2020;11(1):2039. 2020
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Apoptosis characterization in mononuclear blood leukocytes of HIV�patients during dengue acute disease
Authors: A Torrentes-, JC Sánchez-Ar, T Azamor, LS Barbosa, ED Hottz, M Gandini, FA Bozza, RV da Cunha, LM de Oliveir, PV Damasco, EL de Azeredo
Sci Rep, 2020;10(1):6351. 2020
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Sequesterpene Lactones Isolated from a Brazilian Cerrado Plant (Eremanthus spp.) as Anti-Proliferative Compounds, Characterized by Functional and Proteomic Analysis, are Candidates for New Therapeutics in Glioblastoma
Authors: C Izumi, HJ Laure, NG Barbosa, C Hassibe Th, G Aguiar Fer, J Paulo Barr, N Peporine L, J César Rosa
Int J Mol Sci, 2020;21(13):. 2020
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TAK1 regulates the tumor microenvironment through inflammatory, angiogenetic and apoptotic signaling cascades
Authors: SA Scarneo, KW Yang, JR Roques, A Dai, LS Eibschutz, P Hughes, TAJ Haystead
Oncotarget, 2020;11(21):1961-1970. 2020
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Tissue-regenerative potential of the secretome of gamma-irradiated peripheral blood mononuclear cells is mediated via TNFRSF1B-induced necroptosis
Authors: E Simader, L Beer, M Laggner, V Vorstandle, A Gugerell, M Erb, P Kalinina, D Copic, D Moser, A Spittler, E Tschachler, H Jan Ankers, M Mildner
Cell Death Dis, 2019;10(10):729. 2019
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Cytarabine-Resistant FLT3-ITD Leukemia Cells are Associated with TP53 Mutation and Multiple Pathway Alterations-Possible Therapeutic Efficacy of Cabozantinib
Authors: YC Ko, CY Hu, ZH Liu, HF Tien, DL Ou, HF Chien, LI Lin
Int J Mol Sci, 2019;20(5):. 2019
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Heme Oxygenase-1 is a Key Molecule Underlying Differential Response of TW-37-Induced Apoptosis in Human Mucoepidermoid Carcinoma Cells
Authors: IH Yang, CH Ahn, NP Cho, B Jin, W Lee, YC Jung, SD Hong, JA Shin, SD Cho
Molecules, 2019;24(9):. 2019
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Comparison of Radiosensitization by HDAC Inhibitors CUDC-101 and SAHA in Pancreatic Cancer Cells
Authors: S Moertl, S Payer, R Kell, K Winkler, N Anastasov, MJ Atkinson
Int J Mol Sci, 2019;20(13):. 2019
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Kinome expression profiling to target new therapeutic avenues in multiple myeloma
Authors: H de Boussac, A Bruyer, M Jourdan, A Maes, N Robert, C Gourzones, L Vincent, A Seckinger, G Cartron, D Hose, E De Bruyne, A Kassambara, P Pasero, J Moreaux
Haematologica, 2019;0(0):. 2019
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Quercetin Enhances the Anti-Tumor Effects of BET Inhibitors by Suppressing hnRNPA1
Authors: TND Pham, S Stempel, MA Shields, C Spaulding, K Kumar, DJ Bentrem, M Matsangou, HG Munshi
Int J Mol Sci, 2019;20(17):. 2019
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Investigation into the Molecular Mechanisms underlying the Anti-proliferative and Anti-tumorigenesis activities of Diosmetin against HCT-116 Human Colorectal Cancer
Authors: S Koosha, Z Mohamed, A Sinniah, MA Alshawsh
Sci Rep, 2019;9(1):5148. 2019
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Reverse signaling via PD-L1 supports malignant cell growth and survival in classical Hodgkin lymphoma
Authors: S Jalali, T Price-Tros, C Bothun, J Villasboas, HJ Kim, ZZ Yang, AJ Novak, H Dong, SM Ansell
Blood Cancer J, 2019;9(3):22. 2019
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Modeling Snyder-Robinson Syndrome in multipotent stromal cells reveals impaired mitochondrial function as a potential cause for deficient osteogenesis
Authors: AL Ramsay, V Alonso-Gar, C Chaboya, B Radut, B Le, J Florez, C Schumacher, FA Fierro
Sci Rep, 2019;9(1):15395. 2019
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Protein Expression Profiling Identifies Key Proteins and Pathways Involved in Growth Inhibitory Effects Exerted by Guggulsterone in Human Colorectal Cancer Cells
Authors: R Leo, L Therachiyi, SK Siveen, S Uddin, M Kulinski, J Buddenkott, M Steinhoff, AR Krishnanku
Cancers (Basel), 2019;11(10):. 2019
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Semi-Synthetic Ingenol Derivative from Euphorbia tirucalli Inhibits Protein Kinase C Isotypes and Promotes Autophagy and S-phase Arrest on Glioma Cell Lines
Authors: VAO Silva, MN Rosa, A Tansini, O Martinho, A Tanuri, AF Evangelist, A Cruvinel C, JP Lima, LF Pianowski, RM Reis
Molecules, 2019;24(23):. 2019
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PLF-1 (Proliferin-1) Modulates Smooth Muscle Cell Proliferation and Development of Experimental Intimal Hyperplasia
Authors: L Hu, Z Huang, H Ishii, H Wu, S Suzuki, A Inoue, W Kim, H Jiang, X Li, E Zhu, L Piao, G Zhao, Y Lei, K Okumura, GP Shi, T Murohara, M Kuzuya, XW Cheng
J Am Heart Assoc, 2019;8(24):e005886. 2019
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Cytotoxicity of eupatorin in MCF-7 and MDA-MB-231 human breast cancer cells via cell cycle arrest, anti-angiogenesis and induction of apoptosis
Authors: NA Razak, N Abu, WY Ho, NR Zamberi, SW Tan, NB Alitheen, K Long, SK Yeap
Sci Rep, 2019;9(1):1514. 2019
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In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
Authors: N Nordin, SK Yeap, HS Rahman, NR Zamberi, N Abu, NE Mohamad, CW How, MJ Masarudin, R Abdullah, NB Alitheen
Sci Rep, 2019;9(1):1614. 2019
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Mechanisms of Toxicity of Industrially Relevant Silicomanganese Dust on Human 1321N1 Astrocytoma Cells: An In Vitro Study
Authors: YJ Arnoldusse, T Kringlen E, J Samulin Er, I Kero, M Baarnes Er, V Skaug, S Zienolddin
Int J Mol Sci, 2019;20(3):. 2019
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CLEFMA Activates the Extrinsic and Intrinsic Apoptotic Processes through JNK1/2 and p38 Pathways in Human Osteosarcoma Cells
Authors: JS Yang, RC Lin, YH Hsieh, HH Wu, GC Li, YC Lin, SF Yang, KH Lu
Molecules, 2019;24(18):. 2019
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Natural product inspired allicin analogs as novel anti-cancer agents
Authors: I Bhaumik, K Pal, U Debnath, P Karmakar, K Jana, AK Misra
Bioorg. Chem., 2019;86(0):259-272. 2019
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Muscadine grape skin extract inhibits prostate cancer cells by inducing cell-cycle arrest, and decreasing migration through heat shock protein 40
Authors: DN Ignacio, KD Mason, EC Hackett-Mo, C Albanese, L Ringer, WD Wagner, PC Wang, MA Carducci, SK Kachhap, CJ Paller, J Mendonca, L Li-Ying Ch, B Lin, DK Hartle, JE Green, CA Brown, TS Hudson
Heliyon, 2019;5(1):e01128. 2019
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HPV-Mediated Resistance to TNF and TRAIL Is Characterized by Global Alterations in Apoptosis Regulatory Factors, Dysregulation of Death Receptors, and Induction of ROS/RNS
Authors: TK Cabeça, A de Mello A, R Andrette, V de Souza L, MG Morale, F Aguayo, L Termini, LL Villa, AP Lepique, E Boccardo
Int J Mol Sci, 2019;20(1):. 2019
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Effective Treatment of Metastatic Melanoma by Combining MAPK and PI3K Signaling Pathway Inhibitors
Authors: SN Aasen, H Parajuli, T Hoang, Z Feng, K Stokke, J Wang, K Roy, R Bjerkvig, S Knappskog, F Thorsen
Int J Mol Sci, 2019;20(17):. 2019
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DK1 Induces Apoptosis via Mitochondria-Dependent Signaling Pathway in Human Colon Carcinoma Cell Lines In Vitro
Authors: Y Hussin, MNM Aziz, NF Che Rahim, SK Yeap, NE Mohamad, MJ Masarudin, N Nordin, NMA Abd Rahman, CY Yong, MN Akhtar, SNH Zamrus, NB Alitheen
Int J Mol Sci, 2018;19(4):. 2018
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Induction of apoptosis by pinostrobin in human cervical cancer cells: Possible mechanism of action
Authors: A Jaudan, S Sharma, SNA Malek, A Dixit
PLoS ONE, 2018;13(2):e0191523. 2018
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Targeting PDK4 inhibits breast cancer metabolism
Authors: MR Guda, S Asuthkar, CM Labak, AJ Tsung, I Alexandrov, MJ Mackenzie, DV Prasad, KK Velpula
Am J Cancer Res, 2018;8(9):1725-1738. 2018
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6- and 8-Prenylnaringenin, Novel Natural Histone Deacetylase Inhibitors Found in Hops, Exert Antitumor Activity on Melanoma Cells
Authors: S Venturelli, H Niessner, T Sinnberg, A Berger, M Burkard, C Urmann, K Donaubauer, A Böcker, C Leischner, H Riepl, J Frank, UM Lauer, C Garbe, C Busch
Cell. Physiol. Biochem., 2018;51(2):543-556. 2018
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Inhibition of the PI3K but not the MEK/ERK pathway sensitizes human glioma cells to alkylating drugs
Authors: B Haas, V Klinger, C Keksel, V Bonigut, D Kiefer, J Caspers, J Walther, M Wos-Magang, S Weickhardt, G Röhn, M Timmer, R Frötschl, N Eckstein
Cancer Cell Int., 2018;18(0):69. 2018
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Diverse functions of clusterin promote and protect against the development of pulmonary fibrosis
Authors: L Peix, IC Evans, DR Pearce, JK Simpson, TM Maher, RJ McAnulty
Sci Rep, 2018;8(1):1906. 2018
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Curcumin Analog DK1 Induces Apoptosis in Human Osteosarcoma Cells In Vitro through Mitochondria-Dependent Signaling Pathway
Authors: MNM Aziz, Y Hussin, NF Che Rahim, N Nordin, NE Mohamad, SK Yeap, CY Yong, MJ Masarudin, YK Cheah, N Abu, MN Akhtar, NB Alitheen
Molecules, 2018;23(1):. 2018
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Increased sensitivity to apoptosis upon endoplasmic reticulum stress-induced activation of the unfolded protein response in chemotherapy-resistant malignant pleural mesothelioma
Authors: D Xu, SQ Liang, H Yang, U Lüthi, C Riether, S Berezowska, TM Marti, SRR Hall, R Bruggmann, GJ Kocher, RA Schmid, RW Peng
Br. J. Cancer, 2018;0(0):. 2018
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KRas4B-PDE6? complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer
Authors: D Casique-Ag, P Briseño-Dí, P García-Gut, CHG la Rosa, RS Quintero-B, A Rojo-Domín, I Vergara, LA Medina, J Correa-Bas, M Bello, R Hernández-, M Del RocioT, M Vargas
BMC Cancer, 2018;18(1):1299. 2018
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Dual Inhibition of HDAC and tyrosine kinase signaling pathways with CUDC-907 inhibits thyroid cancer growth and metastases
Authors: S Kotian, L Zhang, M Boufraqech, K Gaskins, SK Gara, MM Quezado, N Nilubol, E Kebebew
Clin. Cancer Res., 2017;0(0):. 2017
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Telmisartan inhibits hepatocellular carcinoma cell proliferation in vitro by inducing cell cycle arrest
Authors: K Oura, T Tadokoro, S Fujihara, A Morishita, T Chiyo, E Samukawa, Y Yamana, K Fujita, T Sakamoto, T Nomura, H Yoneyama, H Kobara, H Mori, H Iwama, K Okano, Y Suzuki, T Masaki
Oncol. Rep., 2017;38(5):2825-2835. 2017
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Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
Oncotarget, 2017;8(37):62081-62098. 2017
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Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell
Authors: S Chen, J Tao, F Zhong, Y Jiao, J Xu, Q Shen, H Wang, S Fan, Y Zhang
PLoS ONE, 2017;12(5):e0176501. 2017
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The molecular mechanism of the anticancer effect of Artonin E in MDA-MB 231 triple negative breast cancer cells
Authors: IC Etti, R Abdullah, A Kadir, NM Hashim, SK Yeap, MU Imam, F Ramli, I Malami, KL Lam, U Etti, P Waziri, M Rahman
PLoS ONE, 2017;12(8):e0182357. 2017
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Targeted nanocomplex carrying siRNA against MALAT1 sensitizes glioblastoma to temozolomide
Authors: SS Kim, JB Harford, M Moghe, A Rait, KF Pirollo, EH Chang
Nucleic Acids Res., 2017;0(0):. 2017
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Aspalathin Reverts Doxorubicin-Induced Cardiotoxicity through Increased Autophagy and Decreased Expression of p53/mTOR/p62 Signaling
Authors: R Johnson, S Shabalala, J Louw, AP Kappo, CJF Muller
Molecules, 2017;22(10):. 2017
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Transcriptome Analysis of Gene Expression Provides New Insights into the Effect of Mild Therapeutic Hypothermia on Primary Human Cortical Astrocytes Cultured under Hypoxia
Authors: MM Salman, P Kitchen, MN Woodroofe, RM Bill, AC Conner, PR Heath, MT Conner
Front Cell Neurosci, 2017;11(0):386. 2017
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Discovery of a novel small molecule inhibitor that targets PP2A-?-Catenin signaling and restricts tumor growth and metastasis
Authors: S Maheshwari, AS Rao, A Singh, LR Singh, GR Palnati, RK Arya, SH Cheruvu, S Shahi, T Sharma, S Meena, AK Singh, R Kant, M Riyazuddin, HK Bora, MI Siddiqi, JR Gayen, KV Sashidhara, D Datta
Mol. Cancer Ther., 2017;0(0):. 2017
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MicroRNA-141 enhances anoikis resistance in metastatic progression of ovarian cancer through targeting KLF12/Sp1/survivin axis
Authors: CS Mak, MM Yung, LM Hui, LL Leung, R Liang, K Chen, SS Liu, Y Qin, TH Leung, KF Lee, KK Chan, HY Ngan, DW Chan
Mol. Cancer, 2017;16(1):11. 2017
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Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes
Authors: M Mustapic, E Eitan, JK Werner, ST Berkowitz, MP Lazaropoul, J Tran, EJ Goetzl, D Kapogianni
Front Neurosci, 2017;11(0):278. 2017
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WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
Cell Death Dis, 2017;8(7):e2955. 2017
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Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells
Authors: IC Etti, A Rasedee, NM Hashim, AB Abdul, A Kadir, SK Yeap, P Waziri, I Malami, KL Lim, CJ Etti
Drug Des Devel Ther, 2017;11(0):865-879. 2017
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Enhancing radiosensitization in EphB4 receptor-expressing Head and Neck Squamous Cell Carcinomas
Sci Rep, 2016;6(0):38792. 2016
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Differential effects of Helenalin, an anti-inflammatory sesquiterpene lactone, on the proteome, metabolome and the oxidative stress response in several immune cell types
Authors: Paula Zwicker
Toxicol In Vitro, 2016;40(0):45-54. 2016
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SurR9C84A Protects and Recovers Human Cardiomyocytes from Hypoxia Induced Apoptosis
Authors: Rupinder Kaur Kanwar
Exp. Cell Res., 2016;0(0):. 2016
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Large-scale pharmacological profiling of 3D tumor models of cancer cells
Cell Death Dis, 2016;7(12):e2492. 2016
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Suppression of mir-200 family expression by oncogenic KRAS activation promotes cell survival and epithelial-mesenchymal transition in KRAS-driven cancer
Mol Cell Biol, 2016;0(0):. 2016
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Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells.
Authors: Skolekova S, Matuskova M, Bohac M, Toro L, Demkova L, Gursky J, Kucerova L
Cell Commun Signal, 2016;14(1):4. 2016
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Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment
PLoS ONE, 2016;11(7):e0159013. 2016
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Multiple Active Compounds from Viscum album L. Synergistically Converge to Promote Apoptosis in Ewing Sarcoma
PLoS ONE, 2016;11(9):e0159749. 2016
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Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells
Authors: Weiqiang Zhou
Sci Rep, 2016;6(0):28004. 2016
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MCPIP1 Exogenous Overexpression Inhibits Pathways Regulating MYCN Oncoprotein Stability in Neuroblastoma
Authors: El?bieta Boratyn
J. Cell. Biochem, 2016;0(0):. 2016
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Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
PLoS ONE, 2015;10(4):e0121103. 2015
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Decreased expression of APAF-1 and increased expression of cathepsin B in invasive pituitary adenoma.
Authors: Tanase, Cristian, Albulescu, Radu, Codrici, Elena, Calenic, Bogdan, Popescu, Ionela D, Mihai, Simona, Necula, Laura, Cruceru, Maria Li, Hinescu, Mihail E
Onco Targets Ther, 2015;8(0):81-90. 2015
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GBM Derived Gangliosides Induce T Cell Apoptosis through Activation of the Caspase Cascade Involving Both the Extrinsic and the Intrinsic Pathway.
Authors: Mahata B, Biswas S, Rayman P, Chahlavi A, Ko J, Bhattacharjee A, Li Y, Li Y, Das T, Sa G, Raychaudhuri B, Vogelbaum M, Tannenbaum C, Finke J, Biswas K
PLoS ONE, 2015;10(7):e0134425. 2015
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FAQs
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Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
Reviews for Proteome Profiler Human Apoptosis Array Kit
Average Rating: 4.8 (Based on 12 Reviews)
Have you used Proteome Profiler Human Apoptosis Array Kit?
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COLO 205 cancer cells were drug treated and vehicle and apoptosis proteins analyzed. 3 minute exposure time.
Very nice kit with easy to follow protocol. Good tool for discovery biology. We used to test small molecule induction of apoptosis in breast cancer cells. I would recommend this kit to anyone looking to profile multiple apoptosis pathways at once. Spot placement was very consistent which made analysis easy.
This array is an excellent tool for screening potential apoptosis related targets. However, it would be nice that if more common targets can be included, e.g. tBid, cleaved PARP and cleaved Caspase9.
These are arrays are used to determine the changes in the expression of proteins of different apoptotic pathways. The signal is quantified to detect the changes. Protocol instructions are good and easy to follow.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: BSA
Dilution: 1:1000