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Proteome Profiler Human Apoptosis Array Kit

ARY009 93 Citations 12 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 35 different apoptosis antibodies
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ARY009
Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit.
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Proteome Profiler Human Apoptosis Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of human apoptosis-related proteins. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Key Benefits

  • Detects 35 human apoptosis-related proteins simultaneously
  • Requires no specialized equipment

 

Principle of the Assay

The Proteome Profiler Human Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.

Why Use an Antibody Array to Detect Apoptosis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Antibody Detection Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 
Simultaneously detect the relative levels of these apoptosis-related proteins in a single sample.
Bad TRAIL R1/DR4 PON2
Bax TRAIL R2/DR5 p21/CIP1/CDNK1A
Bcl-2 FADD p27/Kip1
Bcl-x Fas/TNFSF6 Phospho-p53 (S15)
Pro-Caspase-3 HIF-1 alpha Phospho-p53 (S46)
Cleaved Caspase-3 HO-1/HMOX1/HSP32 Phospho-p53 (S392)
Catalase HO-2/HMOX2 Phospho-Rad17 (S635)
cIAP-1 HSP27 Pro-Caspase-3
cIAP-2 HSP60 SMAC/Diablo
Claspin HSP70 Survivin
Clusterin HTRA2/Omi TNF RI/TNFRSF1A
Cytochrome c Livin XIAP

 

Assays for analytes represented in the Human Apoptosis Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems DuoSet® IC ELISA Development Systems (Total) DuoSet® IC ELISA Development Systems (Phospho or Active)
Bad     DYC819  
Bax     DYC820  
Bcl-2     DYC827B  
Bcl-x        
Pro-Caspase-3        
Cleaved Caspase-3 KM300   DYC835  
Catalase        
cIAP-1        
cIAP-2        
Claspin        
Clusterin DCLU00 DY5874 DYC2937  
Cytochrome c DCTC0   DYC897  
TRAIL R1/DR4        
TRAIL R2/DR5        
FADD        
Fas/TNFSF6 DFS00 DY326    
HIF-1alpha     DYC1935  
HO-1/HMOX1/HSP32     DYC3776  
HO-2/HMOX2        
HSP27     DYC1580 DYC2314
HSP60     DYC1800  
HSP70     DYC1663  
HTRA2/Omi        
Livin        
PON2     DYC4344  
p21/CIP1/CDNK1A     DYC1047  
p27/Kip1     DYC2256  
phospho-p53 (S15)     DYC1043 DYC1839
phospho-p53 (S46)     DYC1043 DYC1489
phospho-p53 (S392)     DYC1043 DYC2996
phospho-Rad17 (S635)        
SMAC/Diablo     DYC789  
Survivin DSV00 DYC647    
TNF RI/TNFRSF1A DRT100 DY225    
XIAP     DYC822  

 

Western blotting antibodies for analytes related to the Human Apoptosis Array Kit

Analyte Phospho-Specific Antibodies Affinity Purified Polyclonal Antibodies (Total) Monoclonal Antibodies (Total) Labeled Antibodies (Total)
Bad   AF819 MAB6405  
Bax   AF820 MAB846, MAB883, 2282-MC-100  
Bcl-2   AF810 MAB8272  
Bcl-x   AF800    
Cleaved Caspase-3     MAB835  
Catalase   AF3398    
cIAP-1   AF8181 MAB818  
cIAP-2   AF8171 MAB817, MAB3400, MAB3310 BAF8171
Claspin   AF3310   BAF3310
Clusterin   AF2937 MAB2937 BAF2937
Cytochrome c     MAB897  
Trail R1/DR4   AF347   BAF347
Trail R2/DR5   AF631   BAF631
FADD MAB7047 (S194) AF2938    
Fas/TNFSF6   AF326 MAB142 BAF326
HIF-1alpha   AF1935 MAB1536  
HO-1/HMOX1/ HSP32   AF3776 MAB3776  
HO-2/HMOX2   AF3170 MAB3170  
HSP27 AF2314 (S78/S82) AF1580, AF15801    
HSP60   AF1800 MAB1800  
HSP70   AF1663 MAB1663  
HTRA2/Omi   AF1458 MAB1458  
Livin   AF1161    
PON2   AF4344 MAB4344  
p21/CIP1/CDNK1A   AF1047 MAB1047 BAF1047
p27/Kip1 AF1555 (T157), AF3994 (T198) AF2256 MAB2256  
phospho-p53 (S15) AF1043 (S15), MAB1839 (S15) AF1355 MAB1355 BAF1355, HAF1355
phospho-p53 (S46) AF1489 (S46), MAB1489 (S46) AF1355 MAB1355 BAF1355, HAF1355
phospho-p53 (S392) AF2996 (S392) AF1355 MAB1355 BAF1355, HAF1355
phospho-Rad17 (S635) AF1374 (S635) AF1926    
SMAC/Diablo   AF789, AF7891   BAF789
TNF RI/TNFRSF1A   AF225 MAB225, MAB625 BAF225
XIAP   AF8221 MAB822 BAF8221

R&D Systems' Apoptosis Array Kit is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of expression of many apoptosis-related proteins in a single sample. No specialized equipment is necessary.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

Product Datasheet
COA
SDS

Data Example

Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. View Larger

Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. Figure 1A: K562 human chronic myelogenous leukemia cells.
Figure 1B: THP-1 human acute monocytic leukemia cells.
Figure 1C: SK-Mel-28 human malignant melanoma cells.

Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. View Larger

Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. Jurkat human acute T cell leukemia cells were either untreated or treated with 25 µM etoposide for 6 hours. Etoposide is a topoisomerase II inhibitor that causes caspase-3 cleavage as apoptosis is induced.

Citations for Proteome Profiler Human Apoptosis Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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  1. Chemical, Physical and Biological Triggers of Evolutionary Conserved Bcl-xL-Mediated Apoptosis
    Authors: A Ianevski, E Kulesskiy, K Krpina, G Lou, Y Aman, A Bugai, K Aasumets, Y Akimov, D Bulanova, K Gildemann, AF Arutyunyan, OY Susova, AL Zhuze, P Ji, W Wang, T Holien, M Bugge, E Zusinaite, V Oksenych, H Lysvand, JM Gerhold, M Bjørås, P Johansen, A Waage, CA Heckman, EF Fang, DE Kainov
    Cancers (Basel), 2020;12(6):.  2020
  2. Robustaflavone induces G0/G1 cell cycle arrest and apoptosis in human umbilical vein endothelial cells and exhibits anti-angiogenic effects in vivo
    Authors: WK Sim, JH Park, KY Kim, IS Chung
    Sci Rep, 2020;10(1):11070.  2020
  3. The TUSC2 Tumour Suppressor Inhibits the Malignant Phenotype of Human Thyroid Cancer Cells via SMAC/DIABLO Protein
    Authors: RM Mariniello, FM Orlandella, AE Stefano, PLC Iervolino, G Smaldone, N Luciano, N Cervone, F Munciguerr, S Esposito, P Mirabelli, G Salvatore
    Int J Mol Sci, 2020;21(3):.  2020
  4. Redox Modulation and Induction of Ferroptosis as a New Therapeutic Strategy in Hepatocellular Carcinoma
    Authors: J Lippmann, K Petri, S Fulda, J Liese
    Transl Oncol, 2020;13(8):100785.  2020
  5. Identification of UAP1L1 as tumor promotor in gastric cancer through regulation of CDK6
    Authors: J Qi, S Liu, W Liu, G Cai, G Liao
    Aging (Albany NY), 2020;12(8):6904-6927.  2020
  6. Apoptosis Induced by (-)-Epicatechin in Human Breast Cancer Cells is Mediated by Reactive Oxygen Species
    Authors: F Pereyra-Ve, IM Olivares-C, AG Perez-Ruiz, JP Luna-Arias, JR García-Sán
    Molecules, 2020;25(5):.  2020
  7. Aging-regulated anti-apoptotic long non-coding RNA Sarrah augments recovery from acute myocardial infarction
    Authors: DJ Trembinski, DI Bink, K Theodorou, J Sommer, A Fischer, A van Bergen, CC Kuo, IG Costa, C Schürmann, MS Leisegang, RP Brandes, T Alekseeva, B Brill, A Wietelmann, CN Johnson, A Spring-Con, M Kaulich, S Werfel, S Engelhardt, MN Hirt, K Yorgan, T Eschenhage, L Kirchhof, P Hofmann, N Jaé, I Wittig, N Hamdani, C Bischof, J Krishnan, RH Houtkooper, S Dimmeler, RA Boon
    Nat Commun, 2020;11(1):2039.  2020
  8. Apoptosis characterization in mononuclear blood leukocytes of HIV�patients during dengue acute disease
    Authors: A Torrentes-, JC Sánchez-Ar, T Azamor, LS Barbosa, ED Hottz, M Gandini, FA Bozza, RV da Cunha, LM de Oliveir, PV Damasco, EL de Azeredo
    Sci Rep, 2020;10(1):6351.  2020
  9. Sequesterpene Lactones Isolated from a Brazilian Cerrado Plant (Eremanthus spp.) as Anti-Proliferative Compounds, Characterized by Functional and Proteomic Analysis, are Candidates for New Therapeutics in Glioblastoma
    Authors: C Izumi, HJ Laure, NG Barbosa, C Hassibe Th, G Aguiar Fer, J Paulo Barr, N Peporine L, J César Rosa
    Int J Mol Sci, 2020;21(13):.  2020
  10. TAK1 regulates the tumor microenvironment through inflammatory, angiogenetic and apoptotic signaling cascades
    Authors: SA Scarneo, KW Yang, JR Roques, A Dai, LS Eibschutz, P Hughes, TAJ Haystead
    Oncotarget, 2020;11(21):1961-1970.  2020
  11. Tissue-regenerative potential of the secretome of gamma-irradiated peripheral blood mononuclear cells is mediated via TNFRSF1B-induced necroptosis
    Authors: E Simader, L Beer, M Laggner, V Vorstandle, A Gugerell, M Erb, P Kalinina, D Copic, D Moser, A Spittler, E Tschachler, H Jan Ankers, M Mildner
    Cell Death Dis, 2019;10(10):729.  2019
  12. Cytarabine-Resistant FLT3-ITD Leukemia Cells are Associated with TP53 Mutation and Multiple Pathway Alterations-Possible Therapeutic Efficacy of Cabozantinib
    Authors: YC Ko, CY Hu, ZH Liu, HF Tien, DL Ou, HF Chien, LI Lin
    Int J Mol Sci, 2019;20(5):.  2019
  13. Heme Oxygenase-1 is a Key Molecule Underlying Differential Response of TW-37-Induced Apoptosis in Human Mucoepidermoid Carcinoma Cells
    Authors: IH Yang, CH Ahn, NP Cho, B Jin, W Lee, YC Jung, SD Hong, JA Shin, SD Cho
    Molecules, 2019;24(9):.  2019
  14. Comparison of Radiosensitization by HDAC Inhibitors CUDC-101 and SAHA in Pancreatic Cancer Cells
    Authors: S Moertl, S Payer, R Kell, K Winkler, N Anastasov, MJ Atkinson
    Int J Mol Sci, 2019;20(13):.  2019
  15. Kinome expression profiling to target new therapeutic avenues in multiple myeloma
    Authors: H de Boussac, A Bruyer, M Jourdan, A Maes, N Robert, C Gourzones, L Vincent, A Seckinger, G Cartron, D Hose, E De Bruyne, A Kassambara, P Pasero, J Moreaux
    Haematologica, 2019;0(0):.  2019
  16. Quercetin Enhances the Anti-Tumor Effects of BET Inhibitors by Suppressing hnRNPA1
    Authors: TND Pham, S Stempel, MA Shields, C Spaulding, K Kumar, DJ Bentrem, M Matsangou, HG Munshi
    Int J Mol Sci, 2019;20(17):.  2019
  17. Investigation into the Molecular Mechanisms underlying the Anti-proliferative and Anti-tumorigenesis activities of Diosmetin against HCT-116 Human Colorectal Cancer
    Authors: S Koosha, Z Mohamed, A Sinniah, MA Alshawsh
    Sci Rep, 2019;9(1):5148.  2019
  18. Reverse signaling via PD-L1 supports malignant cell growth and survival in classical Hodgkin lymphoma
    Authors: S Jalali, T Price-Tros, C Bothun, J Villasboas, HJ Kim, ZZ Yang, AJ Novak, H Dong, SM Ansell
    Blood Cancer J, 2019;9(3):22.  2019
  19. Modeling Snyder-Robinson Syndrome in multipotent stromal cells reveals impaired mitochondrial function as a potential cause for deficient osteogenesis
    Authors: AL Ramsay, V Alonso-Gar, C Chaboya, B Radut, B Le, J Florez, C Schumacher, FA Fierro
    Sci Rep, 2019;9(1):15395.  2019
  20. Protein Expression Profiling Identifies Key Proteins and Pathways Involved in Growth Inhibitory Effects Exerted by Guggulsterone in Human Colorectal Cancer Cells
    Authors: R Leo, L Therachiyi, SK Siveen, S Uddin, M Kulinski, J Buddenkott, M Steinhoff, AR Krishnanku
    Cancers (Basel), 2019;11(10):.  2019
  21. Semi-Synthetic Ingenol Derivative from Euphorbia tirucalli Inhibits Protein Kinase C Isotypes and Promotes Autophagy and S-phase Arrest on Glioma Cell Lines
    Authors: VAO Silva, MN Rosa, A Tansini, O Martinho, A Tanuri, AF Evangelist, A Cruvinel C, JP Lima, LF Pianowski, RM Reis
    Molecules, 2019;24(23):.  2019
  22. PLF-1 (Proliferin-1) Modulates Smooth Muscle Cell Proliferation and Development of Experimental Intimal Hyperplasia
    Authors: L Hu, Z Huang, H Ishii, H Wu, S Suzuki, A Inoue, W Kim, H Jiang, X Li, E Zhu, L Piao, G Zhao, Y Lei, K Okumura, GP Shi, T Murohara, M Kuzuya, XW Cheng
    J Am Heart Assoc, 2019;8(24):e005886.  2019
  23. Cytotoxicity of eupatorin in MCF-7 and MDA-MB-231 human breast cancer cells via cell cycle arrest, anti-angiogenesis and induction of apoptosis
    Authors: NA Razak, N Abu, WY Ho, NR Zamberi, SW Tan, NB Alitheen, K Long, SK Yeap
    Sci Rep, 2019;9(1):1514.  2019
  24. In vitro cytotoxicity and anticancer effects of citral nanostructured lipid carrier on MDA MBA-231 human breast cancer cells
    Authors: N Nordin, SK Yeap, HS Rahman, NR Zamberi, N Abu, NE Mohamad, CW How, MJ Masarudin, R Abdullah, NB Alitheen
    Sci Rep, 2019;9(1):1614.  2019
  25. Mechanisms of Toxicity of Industrially Relevant Silicomanganese Dust on Human 1321N1 Astrocytoma Cells: An In Vitro Study
    Authors: YJ Arnoldusse, T Kringlen E, J Samulin Er, I Kero, M Baarnes Er, V Skaug, S Zienolddin
    Int J Mol Sci, 2019;20(3):.  2019
  26. CLEFMA Activates the Extrinsic and Intrinsic Apoptotic Processes through JNK1/2 and p38 Pathways in Human Osteosarcoma Cells
    Authors: JS Yang, RC Lin, YH Hsieh, HH Wu, GC Li, YC Lin, SF Yang, KH Lu
    Molecules, 2019;24(18):.  2019
  27. Natural product inspired allicin analogs as novel anti-cancer agents
    Authors: I Bhaumik, K Pal, U Debnath, P Karmakar, K Jana, AK Misra
    Bioorg. Chem., 2019;86(0):259-272.  2019
  28. Muscadine grape skin extract inhibits prostate cancer cells by inducing cell-cycle arrest, and decreasing migration through heat shock protein 40
    Authors: DN Ignacio, KD Mason, EC Hackett-Mo, C Albanese, L Ringer, WD Wagner, PC Wang, MA Carducci, SK Kachhap, CJ Paller, J Mendonca, L Li-Ying Ch, B Lin, DK Hartle, JE Green, CA Brown, TS Hudson
    Heliyon, 2019;5(1):e01128.  2019
  29. HPV-Mediated Resistance to TNF and TRAIL Is Characterized by Global Alterations in Apoptosis Regulatory Factors, Dysregulation of Death Receptors, and Induction of ROS/RNS
    Authors: TK Cabeça, A de Mello A, R Andrette, V de Souza L, MG Morale, F Aguayo, L Termini, LL Villa, AP Lepique, E Boccardo
    Int J Mol Sci, 2019;20(1):.  2019
  30. Effective Treatment of Metastatic Melanoma by Combining MAPK and PI3K Signaling Pathway Inhibitors
    Authors: SN Aasen, H Parajuli, T Hoang, Z Feng, K Stokke, J Wang, K Roy, R Bjerkvig, S Knappskog, F Thorsen
    Int J Mol Sci, 2019;20(17):.  2019
  31. DK1 Induces Apoptosis via Mitochondria-Dependent Signaling Pathway in Human Colon Carcinoma Cell Lines In Vitro
    Authors: Y Hussin, MNM Aziz, NF Che Rahim, SK Yeap, NE Mohamad, MJ Masarudin, N Nordin, NMA Abd Rahman, CY Yong, MN Akhtar, SNH Zamrus, NB Alitheen
    Int J Mol Sci, 2018;19(4):.  2018
  32. Induction of apoptosis by pinostrobin in human cervical cancer cells: Possible mechanism of action
    Authors: A Jaudan, S Sharma, SNA Malek, A Dixit
    PLoS ONE, 2018;13(2):e0191523.  2018
  33. Targeting PDK4 inhibits breast cancer metabolism
    Authors: MR Guda, S Asuthkar, CM Labak, AJ Tsung, I Alexandrov, MJ Mackenzie, DV Prasad, KK Velpula
    Am J Cancer Res, 2018;8(9):1725-1738.  2018
  34. 6- and 8-Prenylnaringenin, Novel Natural Histone Deacetylase Inhibitors Found in Hops, Exert Antitumor Activity on Melanoma Cells
    Authors: S Venturelli, H Niessner, T Sinnberg, A Berger, M Burkard, C Urmann, K Donaubauer, A Böcker, C Leischner, H Riepl, J Frank, UM Lauer, C Garbe, C Busch
    Cell. Physiol. Biochem., 2018;51(2):543-556.  2018
  35. Inhibition of the PI3K but not the MEK/ERK pathway sensitizes human glioma cells to alkylating drugs
    Authors: B Haas, V Klinger, C Keksel, V Bonigut, D Kiefer, J Caspers, J Walther, M Wos-Magang, S Weickhardt, G Röhn, M Timmer, R Frötschl, N Eckstein
    Cancer Cell Int., 2018;18(0):69.  2018
  36. Diverse functions of clusterin promote and protect against the development of pulmonary fibrosis
    Authors: L Peix, IC Evans, DR Pearce, JK Simpson, TM Maher, RJ McAnulty
    Sci Rep, 2018;8(1):1906.  2018
  37. Curcumin Analog DK1 Induces Apoptosis in Human Osteosarcoma Cells In Vitro through Mitochondria-Dependent Signaling Pathway
    Authors: MNM Aziz, Y Hussin, NF Che Rahim, N Nordin, NE Mohamad, SK Yeap, CY Yong, MJ Masarudin, YK Cheah, N Abu, MN Akhtar, NB Alitheen
    Molecules, 2018;23(1):.  2018
  38. Increased sensitivity to apoptosis upon endoplasmic reticulum stress-induced activation of the unfolded protein response in chemotherapy-resistant malignant pleural mesothelioma
    Authors: D Xu, SQ Liang, H Yang, U Lüthi, C Riether, S Berezowska, TM Marti, SRR Hall, R Bruggmann, GJ Kocher, RA Schmid, RW Peng
    Br. J. Cancer, 2018;0(0):.  2018
  39. KRas4B-PDE6? complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer
    Authors: D Casique-Ag, P Briseño-Dí, P García-Gut, CHG la Rosa, RS Quintero-B, A Rojo-Domín, I Vergara, LA Medina, J Correa-Bas, M Bello, R Hernández-, M Del RocioT, M Vargas
    BMC Cancer, 2018;18(1):1299.  2018
  40. Dual Inhibition of HDAC and tyrosine kinase signaling pathways with CUDC-907 inhibits thyroid cancer growth and metastases
    Authors: S Kotian, L Zhang, M Boufraqech, K Gaskins, SK Gara, MM Quezado, N Nilubol, E Kebebew
    Clin. Cancer Res., 2017;0(0):.  2017
  41. Telmisartan inhibits hepatocellular carcinoma cell proliferation in vitro by inducing cell cycle arrest
    Authors: K Oura, T Tadokoro, S Fujihara, A Morishita, T Chiyo, E Samukawa, Y Yamana, K Fujita, T Sakamoto, T Nomura, H Yoneyama, H Kobara, H Mori, H Iwama, K Okano, Y Suzuki, T Masaki
    Oncol. Rep., 2017;38(5):2825-2835.  2017
  42. Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
    Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
    Oncotarget, 2017;8(37):62081-62098.  2017
  43. Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell
    Authors: S Chen, J Tao, F Zhong, Y Jiao, J Xu, Q Shen, H Wang, S Fan, Y Zhang
    PLoS ONE, 2017;12(5):e0176501.  2017
  44. The molecular mechanism of the anticancer effect of Artonin E in MDA-MB 231 triple negative breast cancer cells
    Authors: IC Etti, R Abdullah, A Kadir, NM Hashim, SK Yeap, MU Imam, F Ramli, I Malami, KL Lam, U Etti, P Waziri, M Rahman
    PLoS ONE, 2017;12(8):e0182357.  2017
  45. Targeted nanocomplex carrying siRNA against MALAT1 sensitizes glioblastoma to temozolomide
    Authors: SS Kim, JB Harford, M Moghe, A Rait, KF Pirollo, EH Chang
    Nucleic Acids Res., 2017;0(0):.  2017
  46. Aspalathin Reverts Doxorubicin-Induced Cardiotoxicity through Increased Autophagy and Decreased Expression of p53/mTOR/p62 Signaling
    Authors: R Johnson, S Shabalala, J Louw, AP Kappo, CJF Muller
    Molecules, 2017;22(10):.  2017
  47. Transcriptome Analysis of Gene Expression Provides New Insights into the Effect of Mild Therapeutic Hypothermia on Primary Human Cortical Astrocytes Cultured under Hypoxia
    Authors: MM Salman, P Kitchen, MN Woodroofe, RM Bill, AC Conner, PR Heath, MT Conner
    Front Cell Neurosci, 2017;11(0):386.  2017
  48. Discovery of a novel small molecule inhibitor that targets PP2A-?-Catenin signaling and restricts tumor growth and metastasis
    Authors: S Maheshwari, AS Rao, A Singh, LR Singh, GR Palnati, RK Arya, SH Cheruvu, S Shahi, T Sharma, S Meena, AK Singh, R Kant, M Riyazuddin, HK Bora, MI Siddiqi, JR Gayen, KV Sashidhara, D Datta
    Mol. Cancer Ther., 2017;0(0):.  2017
  49. MicroRNA-141 enhances anoikis resistance in metastatic progression of ovarian cancer through targeting KLF12/Sp1/survivin axis
    Authors: CS Mak, MM Yung, LM Hui, LL Leung, R Liang, K Chen, SS Liu, Y Qin, TH Leung, KF Lee, KK Chan, HY Ngan, DW Chan
    Mol. Cancer, 2017;16(1):11.  2017
  50. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes
    Authors: M Mustapic, E Eitan, JK Werner, ST Berkowitz, MP Lazaropoul, J Tran, EJ Goetzl, D Kapogianni
    Front Neurosci, 2017;11(0):278.  2017
  51. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017;8(7):e2955.  2017
  52. Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells
    Authors: IC Etti, A Rasedee, NM Hashim, AB Abdul, A Kadir, SK Yeap, P Waziri, I Malami, KL Lim, CJ Etti
    Drug Des Devel Ther, 2017;11(0):865-879.  2017
  53. Enhancing radiosensitization in EphB4 receptor-expressing Head and Neck Squamous Cell Carcinomas
    Sci Rep, 2016;6(0):38792.  2016
  54. Differential effects of Helenalin, an anti-inflammatory sesquiterpene lactone, on the proteome, metabolome and the oxidative stress response in several immune cell types
    Authors: Paula Zwicker
    Toxicol In Vitro, 2016;40(0):45-54.  2016
  55. SurR9C84A Protects and Recovers Human Cardiomyocytes from Hypoxia Induced Apoptosis
    Authors: Rupinder Kaur Kanwar
    Exp. Cell Res., 2016;0(0):.  2016
  56. Large-scale pharmacological profiling of 3D tumor models of cancer cells
    Cell Death Dis, 2016;7(12):e2492.  2016
  57. Suppression of mir-200 family expression by oncogenic KRAS activation promotes cell survival and epithelial-mesenchymal transition in KRAS-driven cancer
    Mol Cell Biol, 2016;0(0):.  2016
  58. Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells.
    Authors: Skolekova S, Matuskova M, Bohac M, Toro L, Demkova L, Gursky J, Kucerova L
    Cell Commun Signal, 2016;14(1):4.  2016
  59. Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment
    PLoS ONE, 2016;11(7):e0159013.  2016
  60. Multiple Active Compounds from Viscum album L. Synergistically Converge to Promote Apoptosis in Ewing Sarcoma
    PLoS ONE, 2016;11(9):e0159749.  2016
  61. Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells
    Authors: Weiqiang Zhou
    Sci Rep, 2016;6(0):28004.  2016
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  1. Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?

    • The proteinase inhibitors are recommended for cell and tissue lysates only.  You do not need them for cell culture supernates.

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Reviews for Proteome Profiler Human Apoptosis Array Kit

Average Rating: 4.8 (Based on 12 Reviews)

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Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 10/20/2020
Application:
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 02/07/2019
Application:
Serum Proteome Profiler Human Apoptosis Array Kit ARY009
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 12/12/2018
Application:

COLO 205 cancer cells were drug treated and vehicle and apoptosis proteins analyzed. 3 minute exposure time.

Serum Proteome Profiler Human Apoptosis Array Kit ARY009
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 12/11/2018
Application:
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 12/11/2018
Application:
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 09/13/2018
Application:

Very nice kit with easy to follow protocol. Good tool for discovery biology. We used to test small molecule induction of apoptosis in breast cancer cells. I would recommend this kit to anyone looking to profile multiple apoptosis pathways at once. Spot placement was very consistent which made analysis easy.

Serum Proteome Profiler Human Apoptosis Array Kit ARY009
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 01/12/2018
Application:
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 10/31/2017
Application:
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 10/31/2017
Application:
Serum Proteome Profiler Human Apoptosis Array Kit ARY009
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 09/07/2017
Application:
Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 01/03/2017
Application:

This array is an excellent tool for screening potential apoptosis related targets. However, it would be nice that if more common targets can be included, e.g. tBid, cleaved PARP and cleaved Caspase9.

Proteome Profiler Human Apoptosis Array Kit
By Anonymous on 10/26/2015
Application: Sample Tested: Tumor cell lyastes

These are arrays are used to determine the changes in the expression of proteins of different apoptotic pathways. The signal is quantified to detect the changes. Protocol instructions are good and easy to follow.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: BSA
Dilution: 1:1000

Serum Proteome Profiler Human Apoptosis Array Kit ARY009