Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of human apoptosis-related proteins. Validated for analyte detection in cell lysates.

Key Benefits

  • Detects 35 human apoptosis-related proteins simultaneously
  • Requires no specialized equipment

 

Principle of the Assay

The Proteome Profiler Human Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.

Why Use an Antibody Array to Detect Apoptosis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Antibody Detection Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 
Simultaneously detect the relative levels of these apoptosis-related proteins in a single sample.
Bad TRAIL R1/DR4 PON2
Bax TRAIL R2/DR5 p21/CIP1/CDNK1A
Bcl-2 FADD p27/Kip1
Bcl-x Fas/TNFSF6 Phospho-p53 (S15)
Pro-Caspase-3 HIF-1 alpha Phospho-p53 (S46)
Cleaved Caspase-3 HO-1/HMOX1/HSP32 Phospho-p53 (S392)
Catalase HO-2/HMOX2 Phospho-Rad17 (S635)
cIAP-1 HSP27 SMAC/Diablo
cIAP-2 HSP60 Survivin
Claspin HSP70 TNF RI/TNFRSF1A
Clusterin HTRA2/Omi XIAP
Cytochrome c Livin  

 

Assays for analytes represented in the Human Apoptosis Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems DuoSet® IC ELISA Development Systems (Total) DuoSet® IC ELISA Development Systems (Phospho or Active) Cell-based ELISA Kits Surveyor IC ELISA Kits
Bad     DYC819      
Bax     DYC820      
Bcl-2     DYC827B      
Bcl-x            
Pro-Caspase-3            
Cleaved Caspase-3 KM300   DYC835      
Catalase            
cIAP-1            
cIAP-2            
Claspin            
Clusterin DCLU00 DY5874 DYC2937      
Cytochrome c DCTC0   DYC897      
TRAIL R1/DR4            
TRAIL R2/DR5            
FADD            
Fas/TNFSF6 DFS00 DY326        
HIF-1alpha     DYC1935      
HO-1/HMOX1/HSP32     DYC3776      
HO-2/HMOX2            
HSP27     DYC1580 DYC2314   SUV2314
HSP60     DYC1800      
HSP70     DYC1663      
HTRA2/Omi            
Livin            
PON2     DYC4344      
p21/CIP1/CDNK1A     DYC1047      
p27/Kip1     DYC2256      
phospho-p53 (S15)     DYC1043 DYC1839    
phospho-p53 (S46)     DYC1043 DYC1489    
phospho-p53 (S392)     DYC1043 DYC2996    
phospho-Rad17 (S635)            
SMAC/Diablo     DYC789      
TNF RI/TNFRSF1A DRT100 DY225        
XIAP     DYC822      

 

Western blotting antibodies for analytes related to the Human Apoptosis Array Kit

Analyte Phospho-Specific Antibodies Affinity Purified Polyclonal Antibodies (Total) Monoclonal Antibodies (Total) Labeled Antibodies (Total)
Bad   AF819 MAB6405  
Bax   AF820 MAB846, MAB883, 2282-MC-100  
Bcl-2   AF810 MAB8272  
Bcl-x   AF800    
Cleaved Caspase-3     MAB835  
Catalase   AF3398    
cIAP-1   AF8181 MAB818  
cIAP-2   AF8171 MAB817, MAB3400, MAB3310 BAF8171
Claspin   AF3310   BAF3310
Clusterin   AF2937 MAB2937 BAF2937
Cytochrome c     MAB897  
Trail R1/DR4   AF347   BAF347
Trail R2/DR5   AF631   BAF631
FADD MAB7047 (S194) AF2938    
Fas/TNFSF6   AF326 MAB142 BAF326
HIF-1alpha   AF1935 MAB1536  
HO-1/HMOX1/ HSP32   AF3776 MAB3776  
HO-2/HMOX2   AF3170 MAB3170  
HSP27 AF2314 (S78/S82) AF1580, AF15801    
HSP60   AF1800 MAB1800  
HSP70   AF1663 MAB1663  
HTRA2/Omi   AF1458 MAB1458  
Livin   AF1161    
PON2   AF4344 MAB4344  
p21/CIP1/CDNK1A   AF1047 MAB1047 BAF1047
p27/Kip1 AF1555 (T157), AF3994 (T198) AF2256 MAB2256  
phospho-p53 (S15) AF1043 (S15), MAB1839 (S15) AF1355 MAB1355 BAF1355, HAF1355
phospho-p53 (S46) AF1489 (S46), MAB1489 (S46) AF1355 MAB1355 BAF1355, HAF1355
phospho-p53 (S392) AF2996 (S392) AF1355 MAB1355 BAF1355, HAF1355
phospho-Rad17 (S635) AF1374 (S635) AF1926    
SMAC/Diablo   AF789, AF7891   BAF789
TNF RI/TNFRSF1A   AF225 MAB225, MAB625 BAF225
XIAP   AF8221 MAB822 BAF8221

 

Data Examples
K562, THP-1 and SK-Mel-28
View Larger Image

Figure 1A: K562 human chronic myelogenous leukemia cells.

Figure 1B: THP-1 human acute monocytic leukemia cells.

Figure 1C: SK-Mel-28 human malignant melanoma cells.

Jurkat human acute T cell leukemia cells
View Larger Image

Figure 2A: Jurkat human acute T cell leukemia cells were either untreated or treated with 25 µM etoposide for 6 hours. Etoposide is a topoisomerase II inhibitor that causes caspase-3 cleavage as apoptosis is induced.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Apoptosis Array Kit
R&D Systems' Human Apoptosis Array Kit (Catalog # ARY009) is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of expression of 35 apoptosis-related proteins in a single sample. No specialized equipment is necessary.
  • Alternate Names:
    Apoptosis Array Kit
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10

  1. MicroRNA-141 enhances anoikis resistance in metastatic progression of ovarian cancer through targeting KLF12/Sp1/survivin axis
    Authors: CS Mak, MM Yung, LM Hui, LL Leung, R Liang, K Chen, SS Liu, Y Qin, TH Leung, KF Lee, KK Chan, HY Ngan, DW Chan
    Mol. Cancer, 2017;16(1):11. 2017
  2. Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells
    Authors: Weiqiang Zhou
    Sci Rep, 2016;6(0):28004. 2016
  3. Suppression of mir-200 family expression by oncogenic KRAS activation promotes cell survival and epithelial-mesenchymal transition in KRAS-driven cancer
    Mol Cell Biol, 2016;0(0):. 2016
  4. Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells.
    Authors: Skolekova S, Matuskova M, Bohac M, Toro L, Demkova L, Gursky J, Kucerova L
    Cell Commun Signal, 2016;14(1):4. 2016
  5. Multiple Active Compounds from Viscum album L. Synergistically Converge to Promote Apoptosis in Ewing Sarcoma
    PLoS ONE, 2016;11(9):e0159749. 2016
  6. Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment
    PLoS ONE, 2016;11(7):e0159013. 2016
  7. Enhancing radiosensitization in EphB4 receptor-expressing Head and Neck Squamous Cell Carcinomas
    Sci Rep, 2016;6(0):38792. 2016
  8. Large-scale pharmacological profiling of 3D tumor models of cancer cells
    Cell Death Dis, 2016;7(12):e2492. 2016
  9. EpCAM Aptamer-siRNA Chimera Targets and Regress Epithelial Cancer.
    Authors: Subramanian N, Kanwar J, Kanwar R, Sreemanthula J, Biswas J, Khetan V, Krishnakumar S
    PLoS ONE, 2015;10(7):e0132407. 2015
  10. Inhibition of Survivin with YM155 Induces Durable Tumor Response in Anaplastic Thyroid Cancer.
    Authors: Mehta A, Zhang L, Boufraqech M, Liu-Chittenden Y, Zhang Y, Patel D, Davis S, Rosenberg A, Ylaya K, Aufforth R, Li Z, Shen M, Kebebew E
    Clin Cancer Res, 2015;21(18):4123-32. 2015
  11. GBM Derived Gangliosides Induce T Cell Apoptosis through Activation of the Caspase Cascade Involving Both the Extrinsic and the Intrinsic Pathway.
    Authors: Mahata B, Biswas S, Rayman P, Chahlavi A, Ko J, Bhattacharjee A, Li Y, Li Y, Das T, Sa G, Raychaudhuri B, Vogelbaum M, Tannenbaum C, Finke J, Biswas K
    PLoS ONE, 2015;10(7):e0134425. 2015
  12. AMP-18 Targets p21 to Maintain Epithelial Homeostasis.
    Authors: Chen P, Li Y, Toback F
    PLoS ONE, 2015;10(4):e0125490. 2015
  13. Decreased expression of APAF-1 and increased expression of cathepsin B in invasive pituitary adenoma.
    Authors: Tanase, Cristian, Albulescu, Radu, Codrici, Elena, Calenic, Bogdan, Popescu, Ionela D, Mihai, Simona, Necula, Laura, Cruceru, Maria Li, Hinescu, Mihail E
    Onco Targets Ther, 2015;8(0):81-90. 2015
  14. Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
    Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
    PLoS ONE, 2015;10(4):e0121103. 2015
  15. Antineoplastic Effects of siRNA against TMPRSS2-ERG Junction Oncogene in Prostate Cancer.
    Authors: Urbinati G, Ali H, Rousseau Q, Chapuis H, Desmaele D, Couvreur P, Massaad-Massade L
    PLoS ONE, 2015;10(5):e0125277. 2015
  16. MicroRNA-155 as an inducer of apoptosis and cell differentiation in Acute Myeloid Leukaemia.
    Authors: Palma, Catalina, Al Sheikha, Dima, Lim, Teck Kha, Bryant, Adam, Vu, Thi Than, Jayaswal, Vivek, Ma, David D
    Mol Cancer, 2014;13(0):79. 2014
  17. High dose ionizing radiation regulates micro RNA and gene expression changes in human peripheral blood mononuclear cells.
    Authors: Beer L, Seemann R, Ristl R, Ellinger A, Kasiri M, Mitterbauer A, Zimmermann M, Gabriel C, Gyongyosi M, Klepetko W, Mildner M, Ankersmit H
    BMC Genomics, 2014;15(0):814. 2014
  18. Targeting cadherin-17 inactivates Ras/Raf/MEK/ERK signaling and inhibits cell proliferation in gastric cancer.
    Authors: Lin Z, Zhang C, Zhang M, Xu D, Fang Y, Zhou Z, Chen X, Qin N, Zhang X
    PLoS ONE, 2014;9(1):e85296. 2014
  19. B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection.
    Authors: Nothelfer K, Arena E, Pinaud L, Neunlist M, Mozeleski B, Belotserkovsky I, Parsot C, Dinadayala P, Burger-Kentischer A, Raqib R, Sansonetti P, Phalipon A
    J Exp Med, 2014;211(6):1215-29. 2014
  20. How liposomal Cisplatin overcomes chemoresistance in ovarian tumour cells.
    Anticancer Res, 2014;34(1):525-30. 2014
  21. Vertical blockade of the IGFR- PI3K/Akt/mTOR pathway for the treatment of hepatocellular carcinoma: the role of survivin.
    Authors: Ou, Da-Liang, Lee, Bin-Shyu, Lin, Liang-In, Liou, Jun-Yang, Liao, Sheng-Ch, Hsu, Chiun, Cheng, Ann-Lii
    Mol Cancer, 2014;13(0):2. 2014
  22. Targeting ovarian cancer and chemoresistance through selective inhibition of sphingosine kinase-2 with ABC294640.
    Authors: White, Martin D, Chan, Lucas, Antoon, James W, Beckman, Barbara
    Anticancer Res, 2013;33(9):3573-9. 2013
  23. Cidofovir: a novel antitumor agent for glioblastoma.
    Authors: Hadaczek P, Ozawa T, Soroceanu L, Yoshida Y, Matlaf L, Singer E, Fiallos E, James C, Cobbs C
    Clin Cancer Res, 2013;19(23):6473-83. 2013
  24. Cold atmospheric plasma (CAP) changes gene expression of key molecules of the wound healing machinery and improves wound healing in vitro and in vivo.
    Authors: Arndt S, Unger P, Wacker E, Shimizu T, Heinlin J, Li Y, Thomas H, Morfill G, Zimmermann J, Bosserhoff A, Karrer S
    PLoS ONE, 2013;8(11):e79325. 2013
  25. The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
    Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
    Cell Death Dis, 2013;4(0):e627. 2013
  26. Cacospongionolide and scalaradial, two marine sesterterpenoids as potent apoptosis-inducing factors in human carcinoma cell lines.
    Authors: De Stefano D, Tommonaro G, Malik SA, Iodice C, De Rosa S, Maiuri MC, Carnuccio R
    PLoS ONE, 2012;7(4):e33031. 2012
  27. Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
    Authors: Stephenson-Famy A, Marks J, Suresh A
    Mol. Endocrinol., 2012;26(2):320-30. 2012
  28. TWEAK affects keratinocyte G2/M growth arrest and induces apoptosis through the translocation of the AIF protein to the nucleus.
    Authors: Sabour Alaoui S, Dessirier V, de Araujo E, Alexaki VI, Pelekanou V, Lkhider M, Stathopoulos EN, Castanas E, Bagot M, Bensussan A, Tsapis A
    PLoS ONE, 2012;7(3):e33609. 2012
  29. NFATc2 is a potential therapeutic target in human melanoma.
    J. Invest. Dermatol., 2012;132(11):2652-60. 2012
  30. Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve.
    Authors: Mohamed SA, Radtke A, Saraei R
    Cardiol Res Pract, 2012;2012(0):165957. 2012
  31. LIM-class homeobox gene Lim1, a novel oncogene in human renal cell carcinoma.
    Authors: Dormoy V, Beraud C, Lindner V, Thomas L, Coquard C, Barthelmebs M, Jacqmin D, Lang H, Massfelder T
    Oncogene, 2011;30(15):1753-63. 2011
  32. Anti-tumor effects of fibroblast growth factor-binding protein (FGF-BP) knockdown in colon carcinoma.
    Authors: Schulze D, Plohmann P, Hobel S, Aigner A
    Mol. Cancer, 2011;10(0):144. 2011
  33. Lapatinib, a preventive/therapeutic agent against mammary cancer, suppresses RTK-mediated signaling through multiple signaling pathways.
    Authors: Li J, Cho YY, Langfald A
    Cancer Prev Res (Phila), 2011;4(8):1190-7. 2011
  34. CXCR3-B can mediate growth-inhibitory signals in human renal cancer cells by down-regulating the expression of heme oxygenase-1.
    Authors: Datta D, Banerjee P, Gasser M
    J. Biol. Chem., 2010;285(47):36842-8. 2010
  35. Bitter melon (Momordica charantia) extract inhibits breast cancer cell proliferation by modulating cell cycle regulatory genes and promotes apoptosis.
    Authors: Ray RB, Raychoudhuri A, Steele R
    Cancer Res., 2010;70(5):1925-31. 2010
  36. Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve.
    Authors: Mohamed SA, Misfeld M, Hanke T, Charitos EI, Bullerdiek J, Belge G, Kuehnel W, Sievers HH
    Ann. Anat., 2010;192(3):145-50. 2010
  37. A proteomic approach to the identification of molecular targets in subsequent apoptosis of HEL cells after diosgenin-induced megakaryocytic differentiation.
    Authors: Cailleteau C, Liagre B, Beneytout JL
    J. Cell. Biochem., 2009;107(4):785-96. 2009
Expand to show all 37 Citations
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  Excellent
  Anonymous 01/03/2017
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Other Experimental Details

Other Experimental DetailsThis array is an excellent tool for screening potential apoptosis related targets. However, it would be nice that if more common targets can be included, e.g. tBid, cleaved PARP and cleaved Caspase9.
 Proteome Profiler Human Apoptosis Array Kit ARY009
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Very Good
  Anonymous 10/26/2015
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 Proteome Profiler Human Apoptosis Array Kit ARY009
: Proteome Profiler Human Apoptosis Array Kit [ARY009]

Summary

Sample TestedTumor cell lyastes

Other Experimental Details

Other Experimental DetailsThese are arrays are used to determine the changes in the expression of proteins of different apoptotic pathways. The signal is quantified to detect the changes. Protocol instructions are good and easy to follow.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: BSA
Dilution: 1:1000

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