Centrin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14909

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Centrin 1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (84%), Chicken (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Centrin 1 Antibody - BSA Free

Western Blot: Centrin 1 Antibody [NBP2-14909]

Western Blot: Centrin 1 Antibody [NBP2-14909]

Western Blot: Centrin 1 Antibody [NBP2-14909] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909]

Immunoprecipitation: Centrin 1 Antibody [NBP2-14909]

Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 ug of Centrin-1 antibody [N1C3]. Western blot analysis was performed using Centrin-1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Centrin 1 Antibody

Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] -

Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - A431 cells were fixed in ice-cold MeOH for 5 min.Green: Centrin 1 stained by Centrin 1 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.
Centrin 1 Antibody

Western Blot: Centrin 1 Antibody [NBP2-14909] -

Western Blot: Centrin 1 Antibody [NBP2-14909] - Sample (30 ug of whole cell lysate)
A: JurKat
12% SDS PAGE
Centrin-1 antibody
NBP2-14909 diluted at 1:1000

Applications for Centrin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Centrin 1

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq]

Alternate Names

calcium binding protein, caltractin, Caltractin isoform 2, CEN1centrin-1, centrin, EF-hand protein, 1, CETN, EF-hand protein

Gene Symbol

CETN1

UniProt

Additional Centrin 1 Products

Product Documents for Centrin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Centrin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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