CEP27 Antibody

Novus Biologicals | Catalog # NBP2-15861

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CEP27 Primary Antibodies »

Product Specifications

Immunogen

Full length human CEP27 Recombinant protein.

Reactivity Notes

Pig (82%), Rhesus Monkey (85%), Bovine (85%).

Localization

Centrosome; Spindle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEP27 Antibody

Western Blot: CEP27 Antibody [NBP2-15861]

Western Blot: CEP27 Antibody [NBP2-15861]

Western Blot: CEP27 Antibody [NBP2-15861] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.
CEP27 Antibody

Immunocytochemistry/ Immunofluorescence: CEP27 Antibody [NBP2-15861] -

HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (NBP2-15861) diluted at 1:500.
Blue: Hoechst 33343 staining.
CEP27 Antibody

Immunocytochemistry/ Immunofluorescence: CEP27 Antibody [NBP2-15861] -

HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (NBP2-15861) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.

Applications for CEP27 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CEP27

HAUS2 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Alternate Names

centrosomal protein 27kDa, Centrosomal protein of 27 kDa, CEP27C15orf25, chromosome 15 open reading frame 25, FLJ10460, HAUS augmin-like complex subunit 2, HAUS augmin-like complex, subunit 2, HsT17025

Gene Symbol

HAUS2

UniProt

Q9NVX0 (Human)

Additional CEP27 Products

Product Documents for CEP27 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CEP27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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