CEP97 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83591

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CEP97 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEP97 Antibody - BSA Free

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591]

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591]

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591] - Staining in human testis and pancreas tissues using anti-CEP97 antibody. Corresponding CEP97 RNA-seq data are presented for the same tissues.
Western Blot: CEP97 Antibody [NBP1-83591]

Western Blot: CEP97 Antibody [NBP1-83591]

Western Blot: CEP97 Antibody [NBP1-83591] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: CEP97 Antibody [NBP1-83591]

Immunocytochemistry/ Immunofluorescence: CEP97 Antibody [NBP1-83591]

Immunocytochemistry/Immunofluorescence: CEP97 Antibody [NBP1-83591] - Staining of human cell line A-431 shows localization to cytosol & microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591]

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591]

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591]

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591]

Immunohistochemistry-Paraffin: CEP97 Antibody [NBP1-83591] - Staining of human testis shows high expression.

Applications for CEP97 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEP97

CEP97 (centrosomal protein of 97 kDa) has been identified as part of a complex with CP110. Together, CP110 and CEP97 have been shown to play a role in the assembly of cilia. Loss of CEP97 or CEP110 promotes cilia formation while forced expression inhibits ciliogenesis. CEP97 is also known as leucine-rich repeat and IQ domain-containing protein 2 or LRRIQ2.

Alternate Names

2810403B08Rik, centrosomal protein 97kDa, centrosomal protein of 97 kDa, Cep97, FLJ23047, FLJ26462, Leucine-rich repeat and IQ domain-containing protein 2, leucine-rich repeats and IQ motif containing 2, LRRIQ2

Entrez Gene IDs

79598 (Human)

Gene Symbol

CEP97

UniProt

Q8IW35 (Human)

Additional CEP97 Products

Product Documents for CEP97 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CEP97 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CEP97 Antibody - BSA Free

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Protocols

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FAQs

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