Chk1 Antibody (2G1D5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80668

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G1D5

Format

Azide and BSA Free
View all Chk1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Chk1 expressed in E. coli. [UniProt# O14757]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk1 Antibody (2G1D5) - Azide and BSA Free

Western Blot: Chk1 Antibody (2G1D5)Azide and BSA Free [NBP2-80668]

Western Blot: Chk1 Antibody (2G1D5)Azide and BSA Free [NBP2-80668]

Western Blot: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668]

Immunocytochemistry/ Immunofluorescence: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668]

Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668]

Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668]

Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase
Western Blot: Chk1 Antibody (2G1D5)Azide and BSA Free [NBP2-80668]

Western Blot: Chk1 Antibody (2G1D5)Azide and BSA Free [NBP2-80668]

Western Blot: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates. Image from the standard format of this antibody.

Applications for Chk1 Antibody (2G1D5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

1:200 - 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chk1

Chk1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by Chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Chk1 Antibody (2G1D5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Chk1 Antibody (2G1D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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