Chk1 [p Ser296] Antibody (SN06-50)

Novus Biologicals | Catalog # NBP2-67711

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN06-50 expressed in HEK293
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Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser296 of human Chk1 (SwissProt: O14757 Human)

Modification

p Ser296

Localization

Chromosome, Cytoplasm, Cytoskeleton, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Chk1 [p Ser296] Antibody (SN06-50)

Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.

Applications for Chk1 [p Ser296] Antibody (SN06-50)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500

Reviewed Applications

Read 1 review rated 5 using NBP2-67711 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chk1

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Chk1 [p Ser296] Antibody (SN06-50)

Certificate of Analysis

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Product Specific Notices for Chk1 [p Ser296] Antibody (SN06-50)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Chk1 [p Ser296] Antibody (SN06-50)

Customer Reviews for Chk1 [p Ser296] Antibody (SN06-50) (1)

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Customer Images

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  • Chk1 [p Ser296] Antibody (SN06-50)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Pancreatic Cancer Cell line
    Species: Human
    Verified Customer | Posted 08/05/2020
    Western Blot: Whole cell lysate from L3.6 PDAC cells prepared using RIPA buffer. Cells were treated with DMSO 48 Hr (left) or Chk1 inhibitor LY2606368 0.78nM 48 Hr (right). Total Chk1 used as loading control (lower bands)
    L3.6 PDAC cell line was treated 48 hours with DMSO or the Chk1 inhibitor LY2606368. Chk1 p Ser296 primary antibody dilution 1:1000 Anti rabbit HRP conjugated secondary dilution 1:2000 Blocking performed using 5% BSA on TBS-Tween solution
    Chk1 [p Ser296] Antibody (SN06-50) NBP2-67711

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Protocols

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FAQs

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