Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5C4

Format

Azide and BSA Free
View all Chk2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk2 Antibody (OTI5C4) - Azide and BSA Free

Western Blot: Chk2 Antibody (OTI5C4)Azide and BSA Free [NBP2-71688]

Western Blot: Chk2 Antibody (OTI5C4)Azide and BSA Free [NBP2-71688]

Western Blot: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.
Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.
Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.
Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.
Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.
Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.
Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688]

Immunohistochemistry: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.
CyTOF-ready: Chk2 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71688] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Chk2 Antibody (OTI5C4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chk2

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Checkpoint Kinase 2

Alternate Names

CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53

Gene Symbol

CHEK2

Additional Chk2 Products

Product Documents for Chk2 Antibody (OTI5C4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Chk2 Antibody (OTI5C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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