Cholecystokinin Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-18225
![Western Blot: Cholecystokinin Antibody [NBP3-18225]](https://resources.rndsystems.com/images/products/Cholecystokinin-Antibody-Western-Blot-NBP3-18225-img0003.jpg "Western Blot: Cholecystokinin Antibody [NBP3-18225]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthesized peptide derived from human Cholecystokinin (Accession P06307), corresponding to amino acid residues I79-R105.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cholecystokinin Antibody - BSA Free
Western Blot: Cholecystokinin Antibody [NBP3-18225]
Western Blot: Cholecystokinin Antibody [NBP3-18225] - Western blot analysis of Cholecystokinin in NIH-3T3 cell lysate. Samples were incubated with Cholecystokinin polyclonal antibody (Product # NBP3-18225).![Immunocytochemistry/ Immunofluorescence: Cholecystokinin Antibody [NBP3-18225]](https://resources.rndsystems.com/images/products/Cholecystokinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18225-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Cholecystokinin Antibody [NBP3-18225]")
Immunocytochemistry/ Immunofluorescence: Cholecystokinin Antibody [NBP3-18225]
Immunocytochemistry/Immunofluorescence: Cholecystokinin Antibody [NBP3-18225] - Immunofluorescent analysis of Cholecystokinin in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25C), incubated with mouse anti-beta tubulin and Cholecystokinin polyclonal antibody (Product # NBP3-18225) using a dilution of 1:200 (1 hr, 37C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).![Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225]](https://resources.rndsystems.com/images/products/Cholecystokinin-Antibody-Immunohistochemistry-Paraffin-NBP3-18225-img0002.jpg "Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225]")
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225]
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225] - Immunohistochemistry analysis of paraffin-embedded Cholecystokinin in human pancreas tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22C), incubated with Cholecystokinin polyclonal antibody (Product # NBP3-18225) using a dilution of 1:200 (1.5 hr, 22C), followed by HRP conjugated goat anti-rabbit.Applications for Cholecystokinin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry-Paraffin
1:200
Western Blot
1:1000 - 1:3000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: CCK
Long Name
Cholecystokinin
Alternate Names
cholecystokinin, MGC117187
Gene Symbol
CCK
Additional CCK Products
Product Documents for Cholecystokinin Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cholecystokinin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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