Cholecystokinin-B R/CCKBR Antibody

Novus Biologicals | Catalog # NB100-2803

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cholecystokinin-B R/CCKBR Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-FDGDSDSDSQSRVRNQ corresponding to internal region according to NP_795344.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cholecystokinin-B R/CCKBR Antibody

Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803]

Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803]

Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - (0.5ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803]

Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803]

Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.
Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803]

Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803]

Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - (6ug/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Cholecystokinin-B R/CCKBR Antibody

Application
Recommended Usage

Immunohistochemistry

4-6 ug/ml

Immunohistochemistry-Paraffin

4-6 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx 48-50kDa band observed in Mouse Pancreas lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1). Primary incubation 1 hour at room temperature. br/> IHC-P: Human pancreas shows staining of the islets of langerhans.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cholecystokinin-B R/CCKBR

CCKBR is a receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Long Name

Cholecystokinin B Receptor

Alternate Names

CCK-B, CCK2R, CCKBR, Cholecystokinin-BR, CholecystokininBR, GASR, Gastrin Receptor

Entrez Gene IDs

887 (Human); 12426 (Mouse); 25706 (Rat)

Gene Symbol

CCKBR

UniProt

P32239 (Human)

Additional Cholecystokinin-B R/CCKBR Products

Product Documents for Cholecystokinin-B R/CCKBR Antibody

Certificate of Analysis

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Product Specific Notices for Cholecystokinin-B R/CCKBR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cholecystokinin-B R/CCKBR Antibody

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Protocols

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