Choline Acetyltransferase/ChAT Antibody

Novus Biologicals | Catalog # NB300-910

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Choline Acetyltransferase/ChAT Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CKEKATRPSQGHQP corresponding to C-Terminus according to NP_066264.3, NP_065574.3, NP_001136405.1.

Marker

Cholinergic Neuronal Marker

Specificity

This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB300-910]

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB300-910]

Western Blot: Choline Acetyltransferase Antibody [NB300-910] - Analysis of Choline Acetyltransferase in Human Placenta lysate using this antibody at 0.03 ug/ml.

Applications for Choline Acetyltransferase/ChAT Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human placenta lysates (calculated MW of 70.5 kDa band according to NP_065574 and NP_066264).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Choline Acetyltransferase/ChAT

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

1103 (Human)

Gene Symbol

CHAT

UniProt

NP_065574 (Human)

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody

Certificate of Analysis

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Product Specific Notices for Choline Acetyltransferase/ChAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Choline Acetyltransferase/ChAT Antibody

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