Choline dehydrogenase Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92326

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Choline dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 245-594 of human CHDH (NP_060867.2). LHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKNGQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPITLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAYQVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGSHIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPTIMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Choline dehydrogenase Antibody - Azide and BSA Free

Choline dehydrogenase Antibody - Azide and BSA Free

Western Blot: Choline dehydrogenase Antibody - Azide and BSA Free [NBP2-92326] -

Western Blot: Choline dehydrogenase Antibody - Azide and BSA Free [NBP2-92326] - Western blot analysis of various lysates, using Choline dehydrogenase Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Negative control (NC):Jurkat
Exposure time: 1s.
Choline dehydrogenase Antibody - Azide and BSA Free

Immunohistochemistry: Choline dehydrogenase Antibody - Azide and BSA Free [NBP2-92326] -

Immunohistochemistry: Choline dehydrogenase Antibody - Azide and BSA Free [NBP2-92326] - Immunohistochemistry analysis of paraffin-embedded Human kidney using Choline dehydrogenase Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Applications for Choline dehydrogenase Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Choline dehydrogenase

Alternate Names

CDH, CHD, choline dehydrogenase, choline dehydrogenase, mitochondrial, EC 1.1.99.1

Gene Symbol

CHDH

Additional Choline dehydrogenase Products

Product Documents for Choline dehydrogenase Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Choline dehydrogenase Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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