Chondroadherin is an approximately 36 kDa secreted glycosaminoglycan that contains nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains. It is a component of the extracellular matrix in cartilage, tendon, bony growth plates, skeletal and cardiac muscle, lung, gastrointestinal tract, pancreas, cerebellum, and eye. It is cleaved by HTRA1/Omi during intervertebral disc degeneration. Chondroadherin functions as an adhesion protein by binding to Integrin alpha 2/beta 1, Collagen II, and Collagen VI. It also promotes complement activation through binding to C1q and Complement Factor H.
Chondroadherin Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-81809
![Western Blot: Chondroadherin AntibodyBSA Free [NBP2-81809]](https://resources.rndsystems.com/images/products/Chondroadherin-Antibody-Western-Blot-NBP2-81809-img0001.jpg "Western Blot: Chondroadherin AntibodyBSA Free [NBP2-81809]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (94%). Backed by our 100% Guarantee.
Applications
Western Blot, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin. The immunogen is located within the last 50 amino acids of Chondroadherin.
Specificity
Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Chondroadherin Antibody - BSA Free
Western Blot: Chondroadherin AntibodyBSA Free [NBP2-81809]
Western Blot: Chondroadherin Antibody [NBP2-81809] - Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 ug/ml.![Immunocytochemistry/ Immunofluorescence: Chondroadherin Antibody - BSA Free [NBP2-81809]](https://resources.rndsystems.com/images/products/Chondroadherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81809-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Chondroadherin Antibody - BSA Free [NBP2-81809]")
Immunocytochemistry/ Immunofluorescence: Chondroadherin Antibody - BSA Free [NBP2-81809]
Immunocytochemistry/Immunofluorescence: Chondroadherin Antibody [NBP2-81809] - Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 ug/ml.Applications for Chondroadherin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry
5 ug/ml
Immunocytochemistry/ Immunofluorescence
5 ug/mL
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chondroadherin
Alternate Names
CHAD, SLRR4A
Gene Symbol
CHAD
Additional Chondroadherin Products
Product Documents for Chondroadherin Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Chondroadherin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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