Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E12

Format

Azide and BSA Free
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CHGA(NP_001266) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Chromogranin A Antibody (OTI2E12) - Azide and BSA Free (NBP2-46282) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Chromogranin A Antibody (OTI2E12) - Azide and BSA Free

Western Blot: Chromogranin A Antibody (OTI2E12)Azide and BSA Free [NBP2-70410]

Western Blot: Chromogranin A Antibody (OTI2E12)Azide and BSA Free [NBP2-70410]

Western Blot: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chromogranin A.
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Chronogranin A.
Immunohistochemistry: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410]

Immunohistochemistry: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410]

Immunohistochemistry: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: Chromogranin A Antibody (OTI2E12)Azide and BSA Free [NBP2-70410]

Western Blot: Chromogranin A Antibody (OTI2E12)Azide and BSA Free [NBP2-70410]

Western Blot: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410] - Analysis of extracts (35ug) from 9 different cell lines by using CHGA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410]

Immunohistochemistry: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410]

Immunohistochemistry: Chromogranin A Antibody (OTI2E12) - Azide and BSA Free [NBP2-70410] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for Chromogranin A Antibody (OTI2E12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (OTI2E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Chromogranin A Antibody (OTI2E12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Chromogranin A Antibody (OTI2E12) - Azide and BSA Free

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    • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
      A: When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.
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