Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1D1
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human CIB1 (1-191aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Novus Biologicals Mouse CIB1 Antibody (1D1) - BSA Free (NBP1-04279) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CIB1 Antibody (1D1) - BSA Free
Western Blot: CIB1 Antibody (1D1)BSA Free [NBP1-04279]
Western Blot: CIB1 Antibody (1D1) [NBP1-04279] - Cell lysates of HT29 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Applications for CIB1 Antibody (1D1) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:1000-1:2000
Reviewed Applications
Read 1 review rated 5 using NBP1-04279 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CIB1
Long Name
Calcium and Integrin Binding 1
Alternate Names
Calmyrin, CIBP, KIP, PRKDCIP, SIP2-28
Gene Symbol
CIB1
UniProt
Additional CIB1 Products
Product Documents for CIB1 Antibody (1D1) - BSA Free
Product Specific Notices for CIB1 Antibody (1D1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for CIB1 Antibody (1D1) - BSA Free (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: HT-29 cellsSpecies: HumanVerified Customer | Posted 12/31/2021HT-29 human colon cancer cell line
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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